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World J Gastroenterol. Mar 14, 2006; 12(10): 1617-1620
Published online Mar 14, 2006. doi: 10.3748/wjg.v12.i10.1617
Association of the HLA-DRB1*0701 allele with perinuclear anti-neutrophil cytoplasmatic antibodies in Mexican patients with severe ulcerative colitis
Jesus K Yamamoto-Furusho, Luis Uscanga-Domínguez, Alondra Lopez-Martinez, Julio Granados
Jesus K Yamamoto-Furusho, Luis Uscanga-Domínguez, Department of Gastroenterology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. México, D F, México Alondra
Lopez-Martinez, Julio Granados. Department of Immunology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. México, D F, México
Author contributions: All authors contributed equally to the work.
Supported by Consejo Nacional de Ciencia y Tecnología CONACYT, NO. 153237
Correspondence to: Dr. Julio Granados; Department of Immunology and Rheumatology,Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Col. Sección XVI. CP 14000 Tlalpan. México, D F, México. julgrate@yahoo.com
Telephone: +52-5-54850080
Received: March 3, 2005
Revised: April 5, 2005
Accepted: May 12, 2005
Published online: March 14, 2006

AIM: To determine the association between the HLA-DRB1 alleles and perinuclear anti-neutrophil cytoplasmatic antibodies (p-ANCA) positive in Mexican patients with ulcerative colitis (UC).

METHODS: Ninety Mexican mestizo patients (45 females) with UC, confirmed by biopsy, were studied. High resolution HLA typing was performed by PCR-SSO reverse dot blot and PCR-SSP. Molecular typing techniques were applied to define HLA-DRB1 alleles. Enzyme-linked immunosorbent assay and immunofluorescence techniques were used to detect p-ANCA.

RESULTS: Forty-eight (53%) UC patients were positive for p-ANCA by ELISA and IF. We found that p-ANCA-positive UC patients had a significantly increased frequency of HLA-DR7 compared with p-ANCA-negative controls (22% vs 5.1%; pC = 0.02, OR = 5.2, CI 95%: 1.06-37.82). Disease activity was scored as severe in 20 patients, moderate in 8, mild in 14 and no activity in the remaining 38 patients according to the Truelove and Witts criteria. Subgroup analysis showed a significantly increased frequency of the HLA-DRB1*07 allele in 15 of 20 UC patients with severe activity of UC and p-ANCA positivity [100% vs 0%; pC = 0.0000001; OR = 35]. No significant differences were found between p-ANCA positive patients, HLA-DR alleles and other clinical features such as extraintestinal manifestations, proctocolectomy and extension.

CONCLUSION: The HLA-DRB1*07 is associated with p-ANCA positive UC Mexican patients.

Keywords: HLA-DR, p-ANCA, Ulcerative colitis, Mexicans