Colorectal Cancer
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 28, 2005; 11(8): 1105-1108
Published online Feb 28, 2005. doi: 10.3748/wjg.v11.i8.1105
Expression of cyclooxygenase-2 in colorectal cancer and its clinical significance
Bin Xiong, Tao-Jiao Sun, Wei-Dong Hu, Fu-Lin Cheng, Min Mao, Yun-Feng Zhou
Bin Xiong, Tao-Jiao Sun, Wei-Dong Hu, Fu-Lin Cheng, Min Mao, Yun-Feng Zhou, Department of Oncology, Affiliated Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Yun-Feng Zhou, Affiliated Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China. binxiong88@yahoo.com
Telephone: +86-27-67813358
Received: February 6, 2004
Revised: February 8, 2004
Accepted: April 5, 2004
Published online: February 28, 2005
Abstract

AIM: To clarify the clinicopathologic significance of COX-2 expression in human colorectal cancer.

METHODS: A total of 128 surgically resected colorectal cancer specimens were immunohistochemically analyzed with the use of anti-COX-2, anti-VEGF and anti-MMP-2 antibodies. The relationship between the cyclooxygenase-2 expression in primary lesions of colorectal cancer and clinicopathologic parameters was evaluated by chi-square test.

RESULTS: Among 128 cases of colorectal cancer, 87 (67.9%) were positive for cyclooxygenase-2. The expression of cyclooxygenase-2 was significantly correlated with the depth of invasion, stage of disease, and metastasis (lymph node and liver). Patients in T3-T4, stages III-IV and with metastasis had much higher expression of cyclooxygenase-2 than ones in T1-T2, stages I-II and without metastasis (P<0.05). Among 45 cases of colorectal cancer with lymph node metastasis, the COX-2- positive rate was 86.7% (39/45) for primary lesions and diffuse cytoplasmic staining for COX-2 protein was detected in cancer cells in 100% of metastatic lesions of the lymph nodes. VEGF expression was detected in 49 tumors (38.3%), and VEGF expression was closely correlated with COX-2 expression. The positive expression rate of VEGF (81.6%) in the cyclooxygenase-2-positive group was higher than that in the cyclooxygenase-2- negative group (18.4%, P<0.05). MMP-2 expression was detected in 88 tumors (68.8%), and MMP-2 expression was closely correlated with COX-2 expression. The positive expression rate of MMP-2 (79.6%) in the positive COX-2 group was higher than that in the negative COX-2 group (20.4%, P<0.05).

CONCLUSION: Cyclooxygenase-2 may be associated with tumor progression by modulating the angiogenesis and cancer cell motility and invasive potential in colorectal cancer and it can be used as a possible biomarker.

Keywords: Cyclooxygenase-2, Colorectal cancer, Immunohistochemical