Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 28, 2005; 11(48): 7615-7619
Published online Dec 28, 2005. doi: 10.3748/wjg.v11.i48.7615
Detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays
Lian-Qun Jin, Jun-Wen Li, Sheng-Qi Wang, Fu-Huan Chao, Xin-Wei Wang, Zheng-Quan Yuan
Lian-Qun Jin, Zheng-Quan Yuan, PLA Center of Disease Control and Prevention, Beijing 100039, China
Jun-Wen Li, Fu-Huan Chao, Xin-Wei Wang, Tianjin Institute of Hygiene and Environmental Medicine, Tianjin 300050, China
Sheng-Qi Wang, Beijing Institute of Radiation Medicine, Beijing 100850, China
Author contributions: All authors contributed equally to the work.
Supported by the National High Technology Research and Development Program of China (863 Program), No. 2002AA2Z2011
Correspondence to: Dr Jun-Wen Li, Tianjin Institute of Hygiene and Environmental Medicine, 1, Da Li Road, Tianjin 300050, China. jinlianqun@sina.com
Telephone: +86-22-84655345 Fax: +86-22-23328809
Received: January 14, 2005
Revised: June 1, 2005
Accepted: June 6, 2005
Published online: December 28, 2005
Abstract

AIM: To detect the common intestinal pathogenic bacteria quickly and accurately.

METHODS: A rapid (<3 h) experimental procedure was set up based upon the gene chip technology. Target genes were amplified and hybridized by oligonucleotide microarrays.

RESULTS: One hundred and seventy strains of bacteria in pure culture belonging to 11 genera were successfully discriminated under comparatively same conditions, and a series of specific hybridization maps corresponding to each kind of bacteria were obtained. When this method was applied to 26 divided cultures, 25 (96.2%) were identified.

CONCLUSION: Salmonella sp., Escherichia coli, Shigella sp., Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, Proteus sp., Bacillus cereus, Vibrio cholerae, Enterococcus faecalis, Yersinia enterocolitica, and Campylobacter jejuni can be detected and identified by our microarrays. The accuracy, range, and discrimination power of this assay can be continually improved by adding further oligonucleotides to the arrays without any significant increase of complexity or cost.

Keywords: Oligonucleotide array; Sequence analysis; Gene chip; Intestines; Microbiology