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World J Gastroenterol. Dec 21, 2005; 11(47): 7536-7540
Published online Dec 21, 2005. doi: 10.3748/wjg.v11.i47.7536
Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27
Jin-Shui Zhu, Long Wang, Guo-Qiang Cheng, Qin Li, Zu-Ming Zhu, Li Zhu
Jin-Shui Zhu, Long Wang, Guo-Qiang Cheng, Qin Li, Zu-Ming Zhu, Li Zhu, Department of Gastroenterology, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, Shanghai 200233, China
Author contributions: All authors contributed equally to the work.
Supported by the Natural Science Foundation of Shanghai, No. 04ZB14072
Correspondence to: Prof. Jin-Shui Zhu, Department of Gastroenterology, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, Shanghai 200233, China. zhujs1803@hotmail.com
Telephone: +86-21-64837019 Fax: +86-21-64837019
Received: February 15, 2005
Revised: April 1, 2005
Accepted: April 2, 2005
Published online: December 21, 2005
Abstract

AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms.

METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells.

RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4±3.12%) compared to the control group (3.12±0.13%, P < 0.01).

CONCLUSION: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.

Keywords: Gastric cancer, Human mutant p27, Cell line MKN-45