Published online Dec 14, 2005. doi: 10.3748/wjg.v11.i46.7345
Revised: September 13, 2005
Accepted: September 16, 2005
Published online: December 14, 2005
AIM: To analyze the effects of NF-κB inhibition by antioxidant pyrrolidine dithiocarbamate (PDTC) or TNF inhibitor pentoxifylline (PTX) on liver regeneration after partial hepatectomy (PH).
METHODS: Saline, PDTC or PTX were injected 1 h before PH and rats were killed at 0.5 and 24 h after PH. Several control groups were used for comparison (injection control groups).
RESULTS: Compared to saline injected controls, NF-κB activation was absent 0.5 h after PH in rats treated with PDTC or PTX. At 24 h after PH, DNA synthesis and PCNA expression were identical in treated and control rats and thus occurred irrespectively of the status of NF-κB activation at 0.5 h. Signal transducer and activator of transcription 3 (Stat3) activation was observed already 0.5 h after PH in saline, PDTC or PTX group and was similar to Stat3 activation in response to injection without PH.
CONCLUSION: These data strongly suggest that (1) NF-κB p65/p50 DNA binding produced in response to PH is not a signal necessary to initiate the liver regeneration, (2) Stat3 activation is a stress response unrelated to the activation of NF-κB. In conclusion, NF-κB activation is not critically required for the process of liver regeneration after PH.