Oral vaccination of mice against rodent malaria with recombinant Lactococcus lactis expressing MSP-119

doi:10.3748/wjg.v11.i44.6975

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Nov 28, 2005 (publication date) through Apr 18, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Research

World J Gastroenterol. Nov 28, 2005; 11(44): 6975-6980
Published online Nov 28, 2005. doi: 10.3748/wjg.v11.i44.6975

Oral vaccination of mice against rodent malaria with recombinant Lactococcus lactis expressing MSP-1₁₉

Zhi-Hong Zhang, Pei-Hong Jiang, Ning-Jun Li, Mi Shi, Weida Huang

Zhi-Hong Zhang, Pei-Hong Jiang, Ning-Jun Li, Mi Shi, Weida Huang, Department of Biochemistry, School of Life Science, Fudan University, Shanghai 200433, China

Author contributions: All authors contributed equally to the work.

Supported by the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), No. 980198

Correspondence to: Weida Huang, Department of Biochemistry, Fudan University, 220 Han Dan Road, Shanghai 200433, China. whuang@fudan.edu.cn

Telephone: +86-21-65643446 Fax: +86-21-55522773

Received: September 18, 2004
Revised: November 15, 2004
Accepted: November 19, 2004
Published online: November 28, 2005

Abstract

AIM: To construct the recombinant Lactococcus lactis as oral delivery vaccination against malaria.

METHODS: The C-terminal 19-ku fragments of MSP1 (MSP-1₁₉) of Plasmodium yoelii 265-BY was expressed in L. lactis and the recombinant L. lactis was administered orally to BALB/c and C57BL/6 mice. After seven interval vaccinations within 4 wk, the mice were challenged with P. yoelii 265-BY parasites of erythrocytic stage. The protective efficacy of recombinant L. lactis was evaluated.

RESULTS: The peak parasitemias in average for the experiment groups of BALB/c and C57BL/6 mice were 0.8±0.4% and 20.8±26.5%, respectively, and those of their control groups were 12.0±0.8% and 60.8±9.6%, respectively. None of the BALB/c mice in both experimental group and control group died during the experiment. However, all the C57BL/6 mice in the control group died within 23 d and all the vaccinated mice survived well.

CONCLUSION: The results imply the potential of recombinant L. lactis as oral delivery vaccination against malaria.

Keywords: Lactococcus lactis, Oral delivery vaccination, Malaria