Review
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 21, 2005; 11(43): 6740-6744
Published online Nov 21, 2005. doi: 10.3748/wjg.v11.i43.6740
Loss of heterozygosity analyzed by single nucleotide polymorphism array in cancer
Hai-Tao Zheng, Zhi-Hai Peng, Sheng Li, Lin He
Hai-Tao Zheng, Zhi-Hai Peng, Department of General Surgery, Shanghai First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China
Sheng Li, Lin He, Shanghai Institutes for Nutrition Science, Chinese Academy of Sciences, Shanghai 200031, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30080016 and 30470977
Correspondence to: Zhi-Hai Peng, MD, Professor, Department of General Surgery, Shanghai First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China. pengpzh@hotmail.com
Telephone: +86-21-63240090-4312 Fax: +86-21-63240090
Received: January 31, 2005
Revised: February 1, 2005
Accepted: February 3, 2005
Published online: November 21, 2005
Abstract

Neoplastic progression is generally characterized by the accumulation of multiple genetic alterations including loss of tumor suppression gene function. Loss of heterozygosity (LOH) has been used to identify genomic regions that harbor tumor suppressor genes and to characterize different tumor types, pathological stages and progression. LOH pattern has been detected by allelotyping using restriction fragment length polymorphism, and later by simple sequence length polymorphisms (SSLPs or microsatellite) for 10 years. This paper reviews the detection of LOH by recently developed single nucleotide polymorphism (SNP) arrays (all analyzed by Affymetrix array); furthermore, its advantage and disadvantage were analyzed in several kinds of cancer.

Keywords: Loss of heterozygosity; Single nucleotide polymorphism; Array; Cancer