Esophageal Cancer
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 14, 2005; 11(42): 6582-6586
Published online Nov 14, 2005. doi: 10.3748/wjg.v11.i42.6582
Adenovirus expressing p27kip1 suppresses growth of established esophageal carcinoma xenografts
Wei-Guo Zhang, Qing-Ming Wu, Jie-Ping Yu, Qiang Tong, Guo-Jian Xie, Xiao-Hu Wang, Sheng-Bao Li
Wei-Guo Zhang, Qing-Ming Wu, Qiang Tong, Guo-Jian Xie, Xiao-Hu Wang, Sheng-Bao Li, Digestive Department, Taihe Hospital, Yunyang Medical College, Shiyan 442000, Hubei Province, China
Jie-Ping Yu, Digestive Department, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Qing-Ming Wu, Digestive Department, Taihe Hospital, Yunyang Medical College, 29 Renmin Nanlu, Shiyan 442000, Hubei Province, China.
Telephone: +86-719-8801431
Received: May 27, 2004
Revised: June 10, 2004
Accepted: June 12, 2004
Published online: November 14, 2005

AIM: To investigate the growth suppression of ade-novirus expressing p27kip1 on established esophageal tumors in nude mice.

METHODS: Esophageal carcinoma xenografts in nude mice were established by tumor tissue mass transplantation. The successfully constructed reco-mbinant adenoviral vectors carrying p27kip1 gene (Ad-p27kip1) were directly injected into the esophageal tumors in nude mice. Compared to control group, the growth curve of tumor was drawn and the growth inhibition rate of tumor was calculated. The histology of tumors was examined by hematoxylin and eosin (H&E) staining. The expression of p27kip1 and survivin was detected in tumors by immunohistochemical technique.

RESULTS: The growth of tumors in gene therapy group with Ad-p27kip1 was obviously suppressed compared to control group (0.42±0.08 g vs 1.17±0.30 g, t=6.39, P<0.01), the inhibition rate of tumor growth reached 64.1%. Pathological detection showed that the tumors in nude mice were poorly differentiated esophageal squamous carcinoma. In addition, the expression of p27kip1 was increased, while the expression of survivin was decreased in tumors after being transfected with Ad-p27kip1.

CONCLUSION: p27kip1 gene therapy mediated by adenovirus vector has a significant inhibitory effect on esophageal carcinoma in vivo. Up-regulated p27kip1 expression and down-regulated survivin expression may be its important mechanisms.

Keywords: p27kip1 gene, Esophageal carcinoma, Xenograft, Nude mice, Survivin gene