Colorectal Cancer
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 21, 2005; 11(39): 6120-6124
Published online Oct 21, 2005. doi: 10.3748/wjg.v11.i39.6120
Genetic alterations and expression of inhibitor of growth 1 in human sporadic colorectal cancer
Li-Sheng Chen, Jian-Bao Wei, Yong-Chun Zhou, Sen Zhang, Jun-Lin Liang, Yun-Fei Cao, Zong-Jiang Tang, Xiao-Long Zhang, Feng Gao
Li-Sheng Chen, Jian-Bao Wei, Yong-Chun Zhou, Sen Zhang, Jun-Lin Liang, Yun-Fei Cao, Zong-Jiang Tang, Xiao-Long Zhang, Feng Gao, Department of Coloproctological Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Guangxi Provincial Scientific Fund for the Returned Overseas Chinese Scholars, No. 0342018 and Key Research Fund from Public Health Bureau of Guangxi, No. 200206
Correspondence to: Dr. Li-Sheng Chen, Department of Coloproctological Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Province, China. clisheng@vip.sina.com
Telephone: +86-771-5356529 Fax: +86-771-5358968
Received: March 31, 2005
Revised: April 15, 2005
Accepted: April 18, 2005
Published online: October 21, 2005
Abstract

AIM: To explore the effect and significance of inhibitor of growth 1 (ING1) gene in carcinogenesis and progression of human sporadic colorectal cancer.

METHODS: mRNA expression, mutation, and loss of heterozygosity (LOH) of ING1 gene in 35 specimens of sporadic colorectal cancer tissues and the matched normal mucous membrane tissues were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), PCR-single strain conformation polymorphism (PCR-SSCP) and PCR-simple sequence length polymorphism (PCR-SSLP) using microsatellite markers, respectively.

RESULTS: The average ratios of light intensities of p33ING1b and p47ING1a mRNA expression in the cancerous tissues were significantly lower than those in normal tissues. The difference between the two mRNA splices was not significant in the matched tissues. In addition, the ratios of light intensities of p33ING1b and p47ING1a mRNA expression in the cancerous tissues of Dukes?stages C and D were significantly lower than those in cancerous tissues of Dukes?stages A and B. However, no mutation of ING1 gene was detected in all 35 cases; only 4 cases of LOH (11.4%) were found.

CONCLUSION: p33ING1b and p47ING1a mRNA expressions are closely related with the carcinogenesis and progression of human sporadic colorectal cancer. No mutation of ING1 gene is found, and there are only few LOH in sporadic colorectal cancers. These might not be the main reasons for the down regulation of ING1 expression. Its low expression may happen in transcription or post-transcription.

Keywords: Colorectal cancer; Inhibitor of growth 1 (ING1); Expression; Mutation; Loss of heterozygosity