Colorectal Cancer
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 28, 2005; 11(36): 5651-5654
Published online Sep 28, 2005. doi: 10.3748/wjg.v11.i36.5651
Effect of SNC19/ST14 gene overexpression on invasion of colorectal cancer cells
Ke-Feng Ding, Li-Feng Sun, Wei-Ting Ge, Han-Guang Hu, Su-Zhan Zhang, Shu Zheng
Ke-Feng Ding, Li-Feng Sun, Surgery of Oncology, The Second Affiliated Hospital, School of Medicine, Zhejinag University, Hangzhou 310009, Zhejiang Province, China
Wei-Ting Ge, Han-Guang Hu, Su-Zhan Zhang, Shu Zheng, Cancer Institute, School of Medicine, Zhejinag University, Hangzhou 310009, Zhejiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30200325
Correspondence to: Professor Shu Zheng, Cancer Institute, School of Medicine, Zhejinag University, 88 Jiefang Road, Hangzhou 310009, Zhejiang Province, China. zhengshu@zju.edu.cn
Telephone: +86-571-87784501 Fax: +86-571-87214404
Received: January 7, 2005
Revised: February 13, 2005
Accepted: February 18, 2005
Published online: September 28, 2005
Abstract

AIM: To study the effect of SNC19/ST14 gene overexpression on invasion in vitro of colorectal cancer cells.

METHODS: The adhesion of SNC19/ST14 gene-transfected cells to ECM was measured by MTT assay. The cell movement was evaluated by wound healing assay. Cell invasion and migration were determined by invasion assay in vitro.

RESULTS: SNC19/ST14 gene overexpression could enhance invasion of colorectal cancer cells in vitro significantly and influence early cell adherence to ECM, but could not change cell movement significantly.

CONCLUSION: SNC19/ST14 gene overexpression increases the local invasion of colorectal cancer cells in vitro.

Keywords: Gene; SNC19/ST14; Colorectal cancer; Invasion