Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 21, 2005; 11(35): 5492-5497
Published online Sep 21, 2005. doi: 10.3748/wjg.v11.i35.5492
Protective effects of non-mitogenic human acidic fibroblast growth factor on hydrogen peroxide-induced damage to cardiomyocytes in vitro
Zhuo-Feng Lin, Xiao-Kun Li, Yuan Lin, Fan Wu, Li-Min Liang, Xiao-Bing Fu
Zhuo-Feng Lin, Department of Rheumatology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, Guangzhou Province, China
Zhuo-Feng Lin, Xiao-Kun Li, Li-Min Liang, Department of Biopharmacy, Pharmacy College, Jinan University, Guangzhou 510632, Guangdong Province, China
Xiao-Kun Li, Biopharmaceutical Research and Development Center, Jinan University, Guangzhou 510632, Guangdong Province, China
Yuan Lin, Department of Ophthalmology, the First Affiliated Hospital of Medical College, Jinan University, Guangzhou 510632, Guangdong Province, China
Fan Wu, Daan Gene Diagnosis Center of Sun Yat-Sen University, Guangzhou 510089, Guangdong Province, China
Xiao-Bing Fu, Wound Healing and Cell Biology Laboratory, Institution of Burns, 304 Hospital, Trauma Center of Postgraduate Medical College, Beijing 100037, China
Author contributions: All authors contributed equally to the work.
Supported by the National 863 Project, No. 2001AA215131 and No. 2002AA2Z3318
Correspondence to: Xiao-Kun Li, MD, Biopharmaceutical Research and Development Center, Jinan University, Guangzhou 510632, Guangdong Province, China. zflwf@sohu.com
Telephone: +86-20-85565109-801
Received: September 18, 2004
Revised: December 23, 2004
Accepted: December 26, 2004
Published online: September 21, 2005
Abstract

AIM: To study the protective effect of non-mitogenic human acidic fibroblast growth factor (FGF) on cardiac oxidative injury in vivo.

METHODS: Ventricular cardiomyocytes were isolated from 1- to 3-d-old neonatal SD mice and cultured in Dulbecco’s minimum essential medium supplemented with 15% fetal bovine serum under an atmosphere of 50 mL/L CO2-95% air at 37 °C, as well as assessed by immunocyto-chemical assay. We constructed the cardiomyocyte injury model by exposure to a certain concentration of H2O2. Cellular viability, superoxide dismutase (SOD) activity, leakage of maleic dialdehyde and anti-apoptosis effect were included to evaluate the cardiac protective effect of non-mitogenic human acidic FGF.

RESULTS: Over 50% of the cardiomyocytes beat spontaneously on the 2nd d of culture and synchronously beat after being cultured for 3 d. Forty-eight hours after plating was completed, the purity of such cultures was 95% myocytes, assessed by an immunocytochemical assay. Cellular viability dramatically decreased with the increasing of the concentration of H2O2. Non-mitogenic human acidic FGF showed significant resistance to the toxic effect of H2O2, significantly increased the cellular viability as well as the activity of SOD, and dramatically decreased the leakage of maleic dialdehyde as well as the cellular apoptosis rate.

CONCLUSION: Hydrogen peroxide shows strong cytotoxicity to the cultured cardiac myocytes, and non-mitogenic human acidic FGF shows strong cardio-protective effect when exposed to a certain concentration of H2O2.

Keywords: Non-mitogenic human acidic fibroblast growth factor, Cardioprotection, Cardiomyocytes, H2O2