Gastric Cancer
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 7, 2005; 11(33): 5129-5135
Published online Sep 7, 2005. doi: 10.3748/wjg.v11.i33.5129
Recurrent chromosomal rearrangements at bands 8q24 and 11q13 in gastric cancer as detected by multicolor spectral karyotyping
Yasuhide Yamashita, Kazuhiro Nishida, Takashi Okuda, Kenichi Nomura, Yosuke Matsumoto, Shoji Mitsufuji, Shigeo Horiike, Hiroyuki Hata, Chohei Sakakura, Akio Hagiwara, Hisakazu Yamagishi, Masafumi Taniwaki
Yasuhide Yamashita, Takashi Okuda, Shoji Mitsufuji, Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Kazuhiro Nishida, Kenichi Nomura, Yosuke Matsumoto, Shigeo Horiike, Masafumi Taniwaki, Department of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Hiroyuki Hata, Second Department of Internal Medicine, Kumamoto University School of Medicine, Kumamoto 860-8555, Japan
Chohei Sakakura, Akio Hagiwara, Hisakazu Yamagishi, Department of Digestive Surgery, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Masafumi Taniwaki, Department of Clinical Molecular Genetics and Laboratory Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Author contributions: All authors contributed equally to the work.
Correspondence to: Masafumi Taniwaki, MD, PhD, Department of Hematology and Oncology, Department of Clinical Molecular Genetics and Laboratory Medicine, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. taniwaki@koto.kpu-m.ac.jp
Telephone: +81-75-251-5651 Fax: +81-75-251-5687
Received: January 19, 2005
Revised: February 15, 2005
Accepted: February 18, 2005
Published online: September 7, 2005
Abstract

AIM: To identify chromosomal translocations specific to gastric cancer (GC), spectral karyotyping (SKY) analysis was performed on established cell lines and cancerous ascitic fluids.

METHODS: SKY analysis of 10 established cell lines and seven cancerous ascitic fluid samples identified recurrent chromosomal breakpoints and translocations in GC, several of which involved chromosomal loci of oncogenes or tumor suppressor genes.

RESULTS: A total of 630 chromosomal breaks were identified. Chromosome no.8 was the most frequently involved in rearrangements (65 breaks), followed by chromosomes no.11 (53), no. 1 (49), no. 7 (46), no. 13 (37), no. 3 (36), no. 17 (33), and no. 20 (29). Frequent breakpoints were detected in 8q24.1 (30 breaks), 11q13 (29), 13q14 (16), 20q11.2 (14), 7q32 (13), 17q11.2 (13), 18q21 (12), 17q23 (9), 18q11.2 (9). SKY analysis identified a total of 242 chromosomal rearrangements including 190 reciprocal and non-reciprocal translocations. The recurrent combinations of chromosomal bands involved in translocations were 8q24.1 and 13q14 (3 cases), 8q24.1 and 11q13 (3), 11q13 and 17q11.2 (2), and 18q11.2 and 20q11.2 (2). Our study validated the ability of SKY to characterize in detail the chromosomal rearrangements in solid tumors and derived cell lines. Moreover, fluorescence in situ hybridization helped to identify the insertions, translocations, and homogeneously staining regions of MYC and CCND1 gene loci.

CONCLUSION: The non-random co-localization of certain cytogenetic bands suggests the importance of chromosomal translocations in gastric carcinogenesis, by serving as landmarks for the cloning of GC causing genes.

Keywords: Gastric cancer; SKY; FISH; Chromosomal translocation