Co-expression of heat shock protein 70 and glucose-regulated protein 94 in human gastric carcinoma cell line BGC-823

doi:10.3748/wjg.v11.i23.3601

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 11, Issue 23

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (2528)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-2) series, Tables (1-1) series.

Item

Count

PDF

238

HTML

2048

Figures (1-2)

123

Tables (1-1)

119

Sum=2528

Jun 21, 2005 (publication date) through Apr 24, 2024

Times Cited of This Article

Times Cited (8)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Brief Reports

World J Gastroenterol. Jun 21, 2005; 11(23): 3601-3604
Published online Jun 21, 2005. doi: 10.3748/wjg.v11.i23.3601

Co-expression of heat shock protein 70 and glucose-regulated protein 94 in human gastric carcinoma cell line BGC-823

Xiao-Ping Wang, Jing Liao, Guo-Zhen Liu, Xing-Cui Wang, Hong-Wei Shang

Xiao-Ping Wang, Jing Liao, Guo-Zhen Liu, Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China

Xing-Cui Wang, Hong-Wei Shang, Laboratory of Morphology, Capital University of Medical Sciences, Beijing 100054, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Xiao-Ping Wang, Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China. wxpphd@yahoo.com.cn

Telephone: +86-10-63051455

Received: June 30, 2004
Revised: July 1, 2004
Accepted: July 11, 2004
Published online: June 21, 2005

Abstract

AIM: To investigate the co-expression and significance of heat shock protein 70 (HSP70) and glucose-regulated protein 94 (grp94) in human gastric carcinoma cell line BGC-823.

METHODS: The expression and localization of HSP70 and grp94 in human gastric carcinoma cell line BGC-823 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. Flow cytometry was used to analyze the correlation between expression of HSP70, grp94 and cell cycle in BGC-823 cell line.

RESULTS: Gastric cancer cell line BGC-823 expressed high level of HSP70 and grp94. The positive rate of HSP70 and grp94 was 84.9±4.94% and 79.6±5.16%, respectively. Both of them were stained in cell plasma. There was a significant difference compared with control group (1.9±0.94%, P<0.01). During the cell cycle, HSP70 and grp94 were continuously expressed in BGC-823.

CONCLUSION: HSP70 and grp94 are highly expressed in human gastric carcinoma BGC-823 cells through the whole cell cycle. There is no relationship between expression of HSP70, grp94 and cell cycle.

Keywords: Heat shock protein 70, Glucose-regulated protein 94, Gastric carcinoma