Mechanism and clinical significance of cyclooxygenase-2 expression in gastric cancer

doi:10.3748/wjg.v11.i21.3240

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Jun 7, 2005; 11(21): 3240-3244
Published online Jun 7, 2005. doi: 10.3748/wjg.v11.i21.3240

Mechanism and clinical significance of cyclooxygenase-2 expression in gastric cancer

Bao-Can Wang, Chang-Qing Guo, Chao Sun, Qiao-Ling Sun, Guo-Yong Liu, Ding-Guo Li

Bao-Can Wang, Chao Sun, Qiao-Ling Sun, Ding-Guo Li, Digestive Department, Xinhua Hospital, Shanghai Second Medical University, Shanghai 200092, China

Chang-Qing Guo, Guo-Yong Liu, Digestive Department, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Ding-Guo Li, Digestive Department, Xinhua Hospital, Shanghai Second Medical University, 1665 Kongjiang Road, Shanghai 200092, China. dingguo-li@hotmail.com

Telephone: +86-21-65790000 Fax: +86-21-65795173

Received: March 23, 2004
Revised: March 24, 2004
Accepted: June 11, 2004
Published online: June 7, 2005

Abstract

AIM: To determine the correlation between methylation status of 5’ CpG island of cyclooxygenase-2 (COX-2) gene and protein expression in gastric cancer tissues for distinguishing the molecular characters of gastric cancers.

METHODS: Methylation status of 5’ CpG island of COX-2 gene was studied by PCR amplification after HpaII and Hha I restrictive enzyme digestion; COX-2 expression was evaluated by immunohistochemical method.

RESULTS: Hpa II and HhaI site were all methylated in 12 normal gastric mucosa tissues, whereas they were demethylated in 77.27% (34/44) and 84.09% (37/44) gastric cancer tissues, respectively. Expression of COX-2 was detected in 68.18% (30/44) gastric cancer tissues, but no expression was found in normal gastric mucosa tissues. In gastric cancer tissues, COX-2 expression was correlated significantly with HpaII site demethylation (29/30 vs 5/14, P<0.001 and HhaI site demethylation (28/30 vs 9/14, P<0.05).

CONCLUSION: The demethylation of 5’ CpG island of gene is necessary for COX-2 expression in human gastric cancer. The expression status of COX-2 may provide theoretical basis for COX-2 targeting gastric cancer treatments.

Keywords: Gastric cancer, Methylation, Cyclooxygenase-2