Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2004; 10(8): 1222-1226
Published online Apr 15, 2004. doi: 10.3748/wjg.v10.i8.1222
Therapeutic polypeptides based on HBcAg18-27 CTL epitope can induce antigen-specific CD8+ CTL-mediated cytotoxicity in HLA-A2 transgenic mice
Tong-Dong Shi, Yu-Zhang Wu, Zheng-Cai Jia, Wei Zhou, Li-Yun Zou
Tong-Dong Shi, Yu-Zhang Wu, Zheng-Cai Jia, Wei Zhou, Li-Yun Zou, Institute of Immunology, Third Military Medical University, Chongqing 400038, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No.30271189, and the National 973 Project, No. 2001CB510001.
Correspondence to: Dr. Tong-Dong Shi, Institute of Immunology, Third Military Medical University, 30 Gaotanyan Street, Chongqing 400038, China.
Telephone: +86-23-68752236-801 Fax: +86-23-68752789
Received: September 23, 2003
Revised: November 27, 2003
Accepted: December 16, 2003
Published online: April 15, 2004

AIM: To explore how to trigger an HLAI-restricted CD8+ T cell response to exogenously synthesized polypeptides in vivo.

METHODS: Three mimetic therapeutic polypeptides based on the immunodominant CTL epitope of HBcAg, the B- epitope of HBV PreS2 region and a common T helper sequence of tetanus toxoid were designed and synthesized with Merrifield’s solid-phase peptide synthesis method. Their immunological properties of inducing TH1 polarization, CD8+ HBV-specific CTL expansion and CD8+ T cell mediated cytotoxicity were investigated in HLA-A2 transgenic mice.

RESULTS: Results demonstrated that the mimetic polypeptides comprised of the immunodominant CTL, B-, and T helper epitopes could trigger specifically and effectively vigorous CD8+ HBV-specific CTL-mediated cytotoxicity and TH1 polarization of T cells in HLA-A2 transgenic mice.

CONCLUSION: A designed universal T helper plus B-epitopes with short and flexible linkers could dramatically improve the immunogenicity of CTL epitopes in vivo. And that the mimetic therapeutic peptides based on the reasonable match of the above CTL, B- and T helper epitopes could be a promising therapeutic peptide vaccine candidate against HBV infection.

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