Viral Hepatitis
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2004; 10(8): 1176-1179
Published online Apr 15, 2004. doi: 10.3748/wjg.v10.i8.1176
Inhibitory effect of oxymatrine on serum hepatitis B virus DNA in HBV transgenic mice
Lun-Gen Lu, Min-De Zeng, Yi-Min Mao, Jing-Yuan Fang, Yu-Lin Song, Zhao-Hui Shen, Ai-Ping Cao
Lun-Gen Lu, Min-De Zeng, Yi-Min Mao, Jing-Yuan Fang, Yu-Lin Song, Zhao-Hui Shen, Ai-Ping Cao, Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China
Author contributions: All authors contributed equally to the work.
Supported by the Key Project of Shanghai Medical Development Foundation, (No: 99ZDI001) and grants from 1999 Youth Liver Diseases Foundation of Chinese Liver Diseases Association
Correspondence to: Lun-Gen Lu, MD, Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China. lulungen@online.sh.cn
Telephone: +86-21-33070834 Fax: +86-21-63364118
Received: August 23, 2003
Revised: September 23, 2003
Accepted: October 7, 2003
Published online: April 15, 2004
Abstract

AIM: To study the inhibitory effect of oxymatrine on serum hepatitis B virus (HBV) DNA in HBV transgenic mice.

METHODS: HBV transgenic mice model was established by microinjection, and identified by HBV DNA integration and replication. Transgenic mice with replicating HBV were divided into 3 groups, and injected with normal saline (group A, n = 9), 50 mg/kg (group B, n = 8) and 100 mg/kg (group C, n = 9) oxymatrine intraperitoneally once a day for 30 d, respectively. Quantitation of serum HBV DNA in HBV transgenic mice was performed by competitive polymerase chain reaction (PCR) in combination with DNA hybridization quantitative detection technique before and after treatment.

RESULTS: Compared with pre-treatment, the serum HBV DNA in group A (F = 1.04, P = 0.9612) and group B (F = 1.13, P = 0.8739) had no changes after treatment. However, in group C serum HBV DNA was significantly decreased (F = 13.97, P = 0.0012). The serum HBV DNA after treatment was lower in group C than in groups B and A (F = 8.65, P = 0.0068; F = 12.35, P = 0.0018; respectively). The serum HBV DNA after treatment was lower in group B than in group A, but there was no statistical significance (F = 1.43, P = 0.652).

CONCLUSION: Oxymatrine has inhibitory effects on serum HBV DNA in HBV transgenic mice.

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