Gastric Cancer
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2004; 10(8): 1132-1136
Published online Apr 15, 2004. doi: 10.3748/wjg.v10.i8.1132
In vitro effects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells
Jia-Yong Chen, Dao-Ming Liang, Ping Gan, Yi Zhang, Jie Lin
Jia-Yong Chen, Dao-Ming Liang, Ping Gan, Yi Zhang, Jie Lin, Department of General Surgery of the Second Affiliated Hospital, Kunming Medical College, Kunming 650101, Yunnan Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Jia-Yong Chen, Department of General Surgery of the Second Affiliated Hospital, Kunming Medical College, Kunming 650101, Yunnan Province, China. chenjiayong776@.hotmail.com
Telephone: +86-871-5352825 Fax: +86-871-5352087
Received: August 10, 2002
Revised: October 23, 2003
Accepted: November 12, 2003
Published online: April 15, 2004
Abstract

AIM: To study the effects of recombinant human growth hormone (rhGH) on growth of human gastric cancer cell line in vitro.

METHODS: Experiment was divided into control group, rhGH group, oxaliplatin (L-OHP) group and rhGH+L-OHP group. Cell inhibitory rate, cell cycle, cell proliferation index (PI) and DNA inhibitory rate of human gastric cancer line BGC823, at different concentrations of rhGH treatment were studied by cell culture, MTT assay and flow cytometry.

RESULTS: The distinctly accelerated effects of rhGH on multiplication of BGC823 cell line were not found in vitro. There was no statistical significance between rhGH group and control group, or between rhGH+L-OHP group and L-OHP group (P > 0.05). The cell growth curve did not rise. Cell inhibitory rate and cells arrested in G0-G1 phase were obviously increased. Meanwhile, cells in S phase and PI were distinctly decreased and DNA inhibitory rate was obviously increased in rhGH+L-OHP group in comparison with control group and rhGH group, respectively (P < 0.01). Cell inhibitory rate showed an increasing trend and PI showed a decreasing trend in rhGH+L-OHP group compared with L-OHP group.

CONCLUSION: In vitro rhGH does not accelerate the multiplication of human gastric cancer cells. It may increase the therapeutic efficacy when it is used in combination with anticancer drugs.

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