Elevated level of spindle checkprotein MAD2 correlates with cellular mitotic arrest, but not with aneuploidy and clinicopathological characteristics in gastric cancer

doi:10.3748/wjg.v10.i22.3240

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Nov 15, 2004; 10(22): 3240-3244
Published online Nov 15, 2004. doi: 10.3748/wjg.v10.i22.3240

Elevated level of spindle checkprotein MAD2 correlates with cellular mitotic arrest, but not with aneuploidy and clinicopathological characteristics in gastric cancer

Chew-Wun Wu, Chin-Wen Chi, Tze-Sing Huang

Chew-Wun Wu, Department of Surgery, Taipei-Veterans General Hospital, Taipei, Taiwan, China

Chin-Wen Chi, Department of Medical Research and Education, Taipei-Veterans General Hospital and Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan, China

Tze-Sing Huang, Division of Cancer Research, National Health Research Institutes, Taipei, Taiwan, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Tze-Sing Huang, Cooperative Laboratory at VGH-Taipei, No. 201, Shih-Pai Road Sec. 2, Taipei 112, Taiwan, China. tshuang@nhri.org.tw

Telephone: +886-2-28712121 Ext. 2641 Fax: +886-2-28748307

Received: February 11, 2004
Revised: February 14, 2004
Accepted: February 26, 2004
Published online: November 15, 2004

Abstract

AIM: To study the relevance of spindle assembly checkprotein MAD2 to cellular mitotic status, aneuploidy and other clinicopathological characteristics in gastric cancer.

METHODS: Western blot analyses were performed to analyze the protein levels of MAD2 and cyclin B1 in the tumorous and adjacent nontumorous tissues of 34 gastric cancer patients. Cell cycle distribution and DNA ploidy of cancer tissues were also determined by flow cytometry. Conventional statistical methods were adopted to determine the relevance of abnormal MAD2 level to mitotic status, aneuploidy and clinicopathological parameters.

RESULTS: Out of 34 gastric cancer patients 25 (74%) exhibited elevated MAD2 levels in their tumorous tissues compared with the corresponding nontumorous tissues. Elevation of MAD2 levels significantly correlated with the increased levels of cyclin B1 expression and G₂/M-phase distribution (P = 0.038 and P = 0.033, respectively), but was not relevant to aneuploidy. The gastric cancer patients with elevated MAD2 levels showed a tendency toward better disease-free and overall survival (P > 0.05). However, no association was found between elevated MAD2 levels and patients’ clinicopathological characteristics.

CONCLUSION: Elevation of MAD2 level is present in 74% of gastric cancer patients, and correlates with increased mitotic checkpoint activity. However, elevation of MAD2 level is not associated with patients aneuploidy and any of the clinicopathological characteristics.

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