Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 1, 2004; 10(21): 3182-3184
Published online Nov 1, 2004. doi: 10.3748/wjg.v10.i21.3182
Pathological characteristics of gastric leiomyoblastoma
Xiao-Feng Huang, Chun-Mei Wang, Bo-Rong Pan, Xiao-Wen Dai, Li Fang, Jia-Ji Yang, Hua Yu, Jun Ren
Xiao-Feng Huang, Chun-Mei Wang, Jia-Ji Yang, Hua Yu, Electron Microscope Center, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Bo-Rong Pan, Jun Ren, Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Xiao-Wen Dai, Li Fang, Department of Pathology, Chinese PLA 117 Hospital, Hangzhou 310013, Zhejiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Foundation of 117 Hospital, No.99008
Correspondence to: Chun-Mei Wang, Electron Microscope Center, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. fmmuem@fmmu.edu.cn
Telephone: +86-29-83374572
Received: February 2, 2004
Revised: March 17, 2004
Accepted: March 24, 2004
Published online: November 1, 2004
Abstract

AIM: To determine the pathological characteristics of gastric leiomyoblastoma.

METHODS: All tissues were obtained during surgery or gastroscopy. Tissue specimens for examination by light microscope were 1 cm × 1 cm × 1 cm in size, fixed in 40 g/L neutral buffered formaldehyde, embedded in paraffin, and stained with hematoxylin and eosin. The fresh tissues obtained for electron microscopy were 1 mm × 1 mm × 1 mm in size, and fixed in phosphate buffered 30 g/L glutaraldehyde, postfixed in 10 g/L osmium tetroxide and dehydrated in graded alcohol, embebbed in Epon 812. Ultrathin sections of 50 nm were stained with uranyl acetate and lead citrate and examined under a JEM-2000 EX transmission electron microscope.

RESULTS: The most important histopathological feature of leiomyoblastoma was the predominance of large, rounded or polygonal cells with characteristic perinuclear clear zone in cytoplasms. The tumor cells arranged in patch, cell junction or junctional complex could be found occasionally between cells under electron microscope. Most of the neoplastic cytoplasms were filled with myofilaments, dense bodies, and dense patches. Rough endoplasmic reticulum dilatated as lakes, and large quantities of protein secretions of intermediate electron density were found in the dilated cisternae. Intracisternal segregation could also be found. The nuclei were round or oval, and anomalous nuclei were found in part of cells.

CONCLUSION: The diagnosis of gastric leiomyoblastoma can be confirmed by electron microscopy. The clear appearance of tumor cells is due to the dilation of rough endoplasmic reticulum, not fat droplets, glycogens or mucus in cytoplasm.

Keywords: $[Keywords]