Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 2004; 10(20): 3006-3010
Published online Oct 15, 2004. doi: 10.3748/wjg.v10.i20.3006
Gene expression profile in liver of hB1F transgenic mice
Shui-Liang Wang, Hua Yang, You-Hua Xie, Yuan Wang, Jian-Zhong Li, Long Wang, Zhu-Gang Wang, Ji-Liang Fu
Shui-Liang Wang, Hua Yang, Jian-Zhong Li, Ji-Liang Fu, Department of Medical Genetics, Second Military Medical University, Shanghai 200433, China
Shui-Liang Wang, PLA Certer for Laboratory Medicine, Fuzhou General Hospital, Fuzhou 350025, Fujian Province, China
You-Hua Xie, Yuan Wang, State Key Laboratory for Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Long Wang, Zhu-Gang Wang, Ji-Liang Fu, Shanghai Nanfang Research Center for Model Organisms, Shanghai 201203, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No.39830360; the National “863” High Technology Research and Development Program of China, No.2001AA221261; the Qi Ming Xing Program from Shanghai Science and Technology Committee, No. 01QA14046
Correspondence to: Professor Ji-Liang Fu, Department of Medical Genetics, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
Telephone: +86-21-25070027 Fax: +86-21-25070027
Received: October 15, 2003
Revised: November 26, 2003
Accepted: December 8, 2003
Published online: October 15, 2004

AIM: To analyze the tissue morphologic phenotype and liver gene expression profile of hB1F transgenic mice.

METHODS: Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed with microchip, and some of the differentially expressed genes were verified with RT-PCR.

RESULTS: The expressions of hB1F were shown in livers from 6 of 7 transgenic mouse lines. The overexpression of hB1F transgene did not cause pathological changes. Expressions of three genes were up-regulated, while down-regulation was observed for 25 genes.

CONCLUSION: The overexpression of hB1F transgene may cause changes of gene expression profiles in the liver of transgenic mice.

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