H Pylori
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 2004; 10(20): 2994-2996
Published online Oct 15, 2004. doi: 10.3748/wjg.v10.i20.2994
Prominent role of γ -glutamyl-transpeptidase on the growth of Helicobacter pylori
Min Gong, Bow Ho
Min Gong, Bow Ho, Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore 117597, Republic of Singapore
Author contributions: All authors contributed equally to the work.
Supported by NMRC Grant No. 0415/2000. Gong Min is a National University of Singapore research scholar
Correspondence to: Bow Ho, Department of Microbiology, Faculty of Medicine, National University of Singapore, Science Drive 2, Singapore 117597, Republic of Singapore. michob@nus.edu.sg
Telephone: +65-68743672 Fax: +65-67766872
Received: October 10, 2003
Revised: November 23, 2003
Accepted: December 3, 2003
Published online: October 15, 2004

AIM: γ -glutamyl transpeptidase (GGT) has been reported as a virulence and colonizing factor of Helicobacter pylori (H pylori). This study examined the effect of GGT on the growth of H pylori.

METHODS: Standard H pylori strain NCTC 11637 and 4 clinical isolates with different levels of GGT activity as measured by an enzymatic assay were used in this study. Growth inhibition and stimulation studies were carried out by culturing H pylori in brain heart infusion broth supplemented with specific GGT inhibitor (L-serine sodium borate complex, SBC) or enhancer (glutathione together with glycyl-glycine), respectively. The growth profiles of H pylori were determined based on viable bacterial count at time interval.

RESULTS: Growth was more profuse for H pylori isolates with higher GGT activity than those present with lower GGT activity. However, in the presence of SBC, growth of H pylori was retarded in a dose dependent manner (P = 0.034). In contrast, higher growth rate was observed when GGT activity was enhanced in the presence of glutathione and glycyl-glycine.

CONCLUSION: Higher GGT activity provides an advantage to the growth of H pylori in vitro. Inhibition of GGT activity by SBC resulted in growth retardation. The study shows that GGT plays an important role on the growth of H pylori.

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