Brief Reports
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 1, 2004; 10(17): 2595-2597
Published online Sep 1, 2004. doi: 10.3748/wjg.v10.i17.2595
Effects of dendritic cells transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response
Hua-Wen Sun, Qi-Bing Tang, Yong-Jun Cheng, Sheng-Qian Zou
Hua-Wen Sun, Department of General Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China
Qi-Bing Tang, Yong-Jun Cheng, Sheng-Qian Zou, Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Hua-Wen Sun, Department of General Surgery, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China. sxshwyq@sina.com
Telephone: +86-27-88317091
Received: October 20, 2003
Revised: December 4, 2003
Accepted: December 16, 2003
Published online: September 1, 2004
Abstract

AIM: To investigate the effects of dendritic cells (DCs) transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response.

METHODS: The wild-type p53 was transduced to DCs with adenovirus, and the DCs were stimulated by gastric cancer lysates. The surface molecules (B7-1, B7-2, MHC-I, MHC-II) of all DCs were detected by FACS, and the ability of the DCs to induce efficient and specific immunological response in anti-51Cr-labeled target cells was studied. BALB/c mice injected with DCs and Mk28 were established, and CTL response in mice immunized with Lywt-p53DC was evaluated. Tumor-bearing mice were treated with Lywt-p53DC.

RESULTS: The surface molecules of Lywt-p53DC had a high expression of B7-1 (86.70% ± 0.07%), B7-2 (18.77% ± 0.08%), MHC-I (87.20% ± 0.05%) and MHC-II (56.70% ± 0.07%); T lymphocytes had a specific CTL lysis ability induced by Lywt-p53DC; the CTL lysis rate was as high as 81%. The immune protection of Lywtp-53DC was obvious, the tumor diameter in Lywtp-53DC group was 3.10 ± 0.31 mm, 2.73 ± 0.23 mm, 3.70 ± 0.07 mm on d 13, 16 and 19, respectively, which were smaller than control, DC, wtp53DC and LyDC group (P < 0.05). Tumor growth rate in Lywtp53DC group was slower than that in other groups (P < 0.05).

CONCLUSION: DCs transfected with wild-type p53 and stimulated by gastric cancer lysates have specific CTL killing activity.

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