Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 2004; 10(16): 2373-2378
Published online Aug 15, 2004. doi: 10.3748/wjg.v10.i16.2373
Intestinal barrier damage caused by trauma and lipopolysaccharide
Lian-An Ding, Jie-Shou Li, You-Sheng Li, Nian-Ting Zhu, Fang-Nan Liu, Li Tan
Lian-An Ding, Jie-Shou Li, You-Sheng Li, Nian-Ting Zhu, Fang-Nan Liu, Li Tan, Jinling Hospital, Nanjing University Medical School, Nanjing 210002, Jiangsu Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Lian-An Ding, Associate Professor, Jinling Hospital, Nanjing University Medical School, 305 East Zhongshan Road, Nanjing 210002, Jiangsu Province, China. dlahaolq@hotmail.com
Telephone: +86-532-2911324 Fax: +86-532-2911840
Received: August 5, 2003
Revised: September 24, 2003
Accepted: October 27, 2003
Published online: August 15, 2004
Abstract

AIM: To investigate the intestinal barrier function damage induced by trauma and infection in rats.

METHODS: Experimental models of surgical trauma and infection were established in rats. Adult Sprague-Dawley rats were divided into 4 groups: control group (n = 8), EN group (n = 10), PN group (n = 9) and Sep group (n = 8). The rats in PN and Sep groups were made into PN models that received isonitrogenous, isocaloric and isovolumic TPN solution during the 7-d period. Rats in EN and Sep groups received laparotomy and cervical catheterization on day 1 and received lipopolysaccharide injection intraperitoneally on d 7. On the 7th day all the animals were gavaged with lactulose and mannitol to test the intestinal permeability. Twenty-four hours later samples were collected and examined.

RESULTS: The inflammatory responses became gradually aggravated from EN group to Sep group. The mucosal structure of small intestine was markedly impaired in PN and Sep groups. There was a low response in IgA level in Sep group when compared with that of EN group. Lipopolysaccharide injection also increased the nitric oxide levels in the plasma of the rats. The intestinal permeability and bacterial translocation increased significantly in Sep group compared with that of control group.

CONCLUSION: One wk of parenteral nutrition causes an atrophy of the intestinal mucosa and results in a moderate inflammatory reaction in the rats. Endotoxemia aggravats the inflammatory responses that caused by laparotomy plus TPN, increases the production of nitric oxide in the body, and damages the intestinal barrier function.

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