Hypermethylation of Syk gene in promoter region associated with oncogenesis and metastasis of gastric carcinoma

doi:10.3748/wjg.v10.i12.1815

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Jun 15, 2004 (publication date) through Apr 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 15, 2004; 10(12): 1815-1818
Published online Jun 15, 2004. doi: 10.3748/wjg.v10.i12.1815

Hypermethylation of Syk gene in promoter region associated with oncogenesis and metastasis of gastric carcinoma

Shui Wang, Yong-Bin Ding, Guo-Yu Chen, Jian-Guo Xia, Zhen-Yan Wu

Shui Wang, Yong-Bin Ding, Guo-Yu Chen, Jian-Guo Xia, Zhen-Yan Wu, Department of General Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China

Author contributions: All authors contributed equally to the work.

Supported by the Science Fund of Department of Science and Technology of Jiangsu Province. 03KJD320138

Correspondence to: Yong-Bin Ding, Department of General Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. njdyb@sina.com

Telephone: +86-25-86563750 Fax: +86-25-86563750

Received: October 24, 2003
Revised: December 1, 2003
Accepted: December 8, 2003
Published online: June 15, 2004

Abstract

AIM: To investigate the rrelationship between methylation of Syk (spleen tyrosine kinase) gene in promoter region and oncogenesis, metastasis of gastric carcinoma. The relation between silencing of the Syk gene and methylation of Syk promoter region was also studied.

METHODS: By using methylation-specific PCR (MSP) technique, the methylation of Syk promoter region in specimens from 61 gastric cancer patients (tumor tissues and adjacent normal tissues) was detected. Meanwhile, RT-PCR was used to analyse syk expression exclusively.

RESULTS: The expression of the Syk gene was detected in all normal gastric tissues. Syk expression in gastric carcinoma was lower in 14 out of 61 gastric cancer samples than in adjacent normal tissues (χ² = 72.3, P < 0.05). No methylation of Syk promoter was found in adjacent normal tissues. hypermethylation of Syk gene in promoter was detected 21 cases in 61 gastric carcinoma patients. The rate of methylation of Syk promoter in gastric carcinoma was higher than that in adjacent normal tissues (χ² = 25.1, P < 0.05). In 31 patients with lymph node metastasis, 17 were found with Syk promoter methylation. A significant difference was noted between two groups (χ² = 11.4,P < 0.05).

CONCLUSION: Hypermethylation leads to silencing of the Syk gene in human gastric carcinoma. Methylation of Syk promoter is correlated to oncogenesis and metastasis of gastric carcinoma. Syk is considered to be a potential tumor suppressor and anti-metastasis gene in human gastric cancer.

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