Yuan JP, Li T, Li ZH, Yang GZ, Hu BY, Shi XD, Shi TL, Tong SQ, Guo XK. mRNA expression profiling reveals a role of Helicobacter pylori vacuolating toxin in escaping host defense. World J Gastroenterol 2004; 10(10): 1528-1532 [PMID: 15133867 DOI: 10.3748/wjg.v10.i10.1528]
Corresponding Author of This Article
Xiao-Kui Guo, Department of Microbiology and Parasitology, Shanghai Second Medical University, 280 Chongqingnan Road, Shanghai 200025, China. xkguo@shsmu.edu.cn
Article-Type of This Article
Brief Reports
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Jian-Ping Yuan, Zhen-Hong Li, Gui-Zhen Yang, Bao-Yu Hu, Xiao-Dong Shi, Shan-Qing Tong, Xiao-Kui Guo, Tao Li(equal contributor), Department of Medical Microbiology and Parasitology, Shanghai Second Medical University, Shanghai 200025, China
Tie-Liu Shi, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China
Author contributions: All authors contributed equally to the work.
Supported by the State Ministry of Education Research Foundation for Returned Overseas Chinese Scholars Abroad (2001) 498
Correspondence to: Xiao-Kui Guo, Department of Microbiology and Parasitology, Shanghai Second Medical University, 280 Chongqingnan Road, Shanghai 200025, China. xkguo@shsmu.edu.cn
Telephone: +86-21-64671226 Fax: +86-21-64671226
Received: October 31, 2003 Revised: January 10, 2004 Accepted: February 1, 2004 Published online: May 15, 2004
Abstract
AIM: To study the immune response of host to Helicobacter pylori VacA.
METHODS: The monocyte/macrophage-like U937 cells were infected with Helicobacter pylori vacA-positive strain NCTC 11638 or isogenic vacA-negative mutant. Differentially expressed genes were identified at 2, 6, 10, and 24 h post-infection by cDNA microarray. Differential expressions of some genes were confirmed by Northern blot.
RESULTS: More than 100 genes altered their mRNA expression at different time points respectively, many of which were identified to be related to immune evasion.
CONCLUSION: VacA is a crucial element for H pylori to escape from host immune defense by means of differentially regulating the expression of some related genes. These genes, previously known or unknown to be involved in the mechanism of immune evasion, deserve further investigation to unearth much more information complicated in the immune response.
