|
1
|
Wu R, Song YF, Gu Y, Li J, Niu Z, Zhou DH. A chemiluminescence immunoassay for detection of Toxoplasma gondii antibody in porcine based on GRA6 and SAG1. Vet Parasitol 2025; 335:110437. [PMID: 40023974 DOI: 10.1016/j.vetpar.2025.110437] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/15/2024] [Revised: 02/20/2025] [Accepted: 02/26/2025] [Indexed: 03/04/2025]
Abstract
Toxoplasmosis is a zoonotic protozoan disease caused by Toxoplasma gondii. Swine serve as intermediate hosts in the transmission of T. gondii, rendering the detection of this pathogen in swine populations is essential for public health. The chemiluminescence immunoassay (CLIA) is a highly regarded quantitative serological technique recognized for its exceptional sensitivity, specificity, and extensive linear range, thereby facilitating its application in the detection of swine diseases. In this study, we developed CLIA assays using GRA6 (dense granule protein 6), SAG1 (surface antigens 1), and GRA6-SAG1 antigens to detect T. gondii in pigs, achieving results in just 20 minutes. Testing 148 serum samples revealed that the GRA6-SAG1 CLIA had the highest diagnostic performance, with a sensitivity of 97.96 %, specificity of 100.00 %, and an intra-batch coefficient of variation below 8.00 %. Furthermore, analysis of 300 serum samples collected from swine in Fujian province showed the positive rates the T. gondii detection by the GRA6, SAG1, and GRA6-SAG1 CLIA assays is 66.00 %, 68.67 %, and 74.67 %, respectively. The T. gondiii positive rates were higher than those of commercial ELISA kits A (61.00 %) and B (16.33 %) as well as indirect hemagglutination (IHA) kit C (8.33 %). The GRA6-SAG1 CLIA developed in this study presents a rapid, specific, sensitive, and stable method for the detection of antibodies against T. gondii, holding significant potential for the accurate diagnosis of toxoplasmosis in swine.
Collapse
Affiliation(s)
- Ran Wu
- Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
| | - Ya-Fei Song
- Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
| | - Yanlong Gu
- Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
| | - Jiang Li
- Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
| | - Zhipeng Niu
- Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
| | - Dong-Hui Zhou
- Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
| |
Collapse
|
|
2
|
Zheng C, Cui M, Zhang Y, Liu L, Li W, Zhang J, Ji M, Chen W, Jiang W, Wang P, Zhang W. Universal sulfatase-based chemiluminescence biosensing platform: Validation via AFP detection in clinical blood samples. Biosens Bioelectron 2025; 267:116771. [PMID: 39265427 DOI: 10.1016/j.bios.2024.116771] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/24/2024] [Revised: 08/14/2024] [Accepted: 09/09/2024] [Indexed: 09/14/2024]
Abstract
Enzyme-catalyzed chemiluminescence has been widely used in the field of biomedicine, especially in the test kit for various biomarkers. However, the currently reported enzyme-catalyzed chemiluminescence systems suffered from the addition of oxidizing substances, short emission wavelength, and susceptibility to interference by autofluorescence. In this paper, a universal sulfatase-based chemiluminescence system with NIR was developed, in which the designed substrate QM-CF could be transformed into 1,2-dioxetane derivate in the presence of sulfatase and oxygen. This system exhibited long emission wavelengths and CL half-time, a high signal-noise ratio, and without other additives. Importantly, the sulfatase-based chemiluminescence enzyme-linked immunoassay platform was successfully constructed and could be generally applied to detect biomarkers. As a proof of concept, the sulfatase-labeled AFP antibody and substate QM-CF were conveniently suitable for commercial AFP test kits, leading to satisfactory detection results of AFP in clinical blood samples.
Collapse
Affiliation(s)
- Cheng Zheng
- Health Commission of Henan Province Key Laboratory for Precision Diagnosis and Treatment of Pediatric Tumor, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou, 450018, China; Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China; Department of Nuclear Medicine & Laboratory of Clinical Nuclear Medicine, West China Hospital, Sichuan University, Chengdu, 610044, China
| | - Mengyuan Cui
- School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China
| | - Yingyu Zhang
- Henan Key Laboratory of Rare Diseases, Endocrinology and Metabolism Center, the First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, 471003, China
| | - Li Liu
- Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China
| | - Wenqing Li
- Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China
| | - Jiaqi Zhang
- Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China
| | - Min Ji
- School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China
| | - Wei Chen
- Department of Nuclear Medicine & Laboratory of Clinical Nuclear Medicine, West China Hospital, Sichuan University, Chengdu, 610044, China
| | - Wen Jiang
- Department of Pathology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210009, China.
| | - Peng Wang
- Health Commission of Henan Province Key Laboratory for Precision Diagnosis and Treatment of Pediatric Tumor, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou, 450018, China; Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China.
| | - Wancun Zhang
- Health Commission of Henan Province Key Laboratory for Precision Diagnosis and Treatment of Pediatric Tumor, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou, 450018, China.
| |
Collapse
|
|
3
|
Metternich JT, Patjoshi SK, Kistwal T, Kruss S. High-Throughput Approaches to Engineer Fluorescent Nanosensors. ADVANCED MATERIALS (DEERFIELD BEACH, FLA.) 2025; 37:e2411067. [PMID: 39533494 PMCID: PMC11707575 DOI: 10.1002/adma.202411067] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/29/2024] [Revised: 10/23/2024] [Indexed: 11/16/2024]
Abstract
Optical sensors are powerful tools to identify and image (biological) molecules. Because of their optoelectronic properties, nanomaterials are often used as building blocks. To transduce the chemical interaction with the analyte into an optical signal, the interplay between surface chemistry and nanomaterial photophysics has to be optimized. Understanding these aspects promises major opportunities for tailored sensors with optimal performance. However, this requires methods to create and explore the many chemical permutations. Indeed, many current approaches are limited in throughput. This affects the chemical design space that can be studied, the application of machine learning approaches as well as fundamental mechanistic understanding. Here, an overview of selection-limited and synthesis-limited approaches is provided to create and identify molecular nanosensors. Bottlenecks are discussed and opportunities of non-classical recognition strategies are highlighted such as corona phase molecular recognition as well as the requirements for high throughput and scalability. Fluorescent carbon nanotubes are powerful building blocks for sensors and their huge chemical design space makes them an ideal platform for high throughput approaches. Therefore, they are the focus of this article, but the insights are transferable to any nanosensor system. Overall, this perspective aims to provide a fresh perspective to overcome current challenges in the nanosensor field.
Collapse
Affiliation(s)
- Justus T. Metternich
- Fraunhofer Institute for Microelectronic Circuits and SystemsFinkenstrasse 6147057DuisburgGermany
- Department of ChemistryRuhr‐University BochumUniversitätsstrasse 15044801BochumGermany
| | - Sujit K. Patjoshi
- Department of ChemistryRuhr‐University BochumUniversitätsstrasse 15044801BochumGermany
| | - Tanuja Kistwal
- Department of ChemistryRuhr‐University BochumUniversitätsstrasse 15044801BochumGermany
| | - Sebastian Kruss
- Fraunhofer Institute for Microelectronic Circuits and SystemsFinkenstrasse 6147057DuisburgGermany
- Department of ChemistryRuhr‐University BochumUniversitätsstrasse 15044801BochumGermany
- Center for Nanointegration Duisburg‐Essen (CENIDE)Carl‐Benz‐Strasse 19947057DuisburgGermany
| |
Collapse
|
|
4
|
Pieńkos M, Zadykowicz B. What Other Than Acridinium Esters? Computational Search for New Acridinium-Based Chemiluminogens. Int J Mol Sci 2023; 24:ijms24119468. [PMID: 37298422 DOI: 10.3390/ijms24119468] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/09/2023] [Revised: 05/24/2023] [Accepted: 05/26/2023] [Indexed: 06/12/2023] Open
Abstract
The rapid increase in disease prevalence in the world makes it extremely important to search for new or develop existing diagnostic methods, for example, chemiluminescent labeling used in immunodiagnostics. At present, acridinium esters are willingly used as chemiluminogenic fragments of labels. However, the search for new chemiluminogens that are particularly efficient is the main task of our studies. The density functional theory (DFT) and time-dependent (TD) DFT methods were used to obtain thermodynamic and kinetic results concerning the chemiluminescence and competitive dark reactions, which indicated whether some of the scrutinized derivatives have better characteristics than the chemiluminogens used so far. Synthesis of these candidates for efficient chemiluminogens, followed by studies of their chemiluminescent properties, and ultimately in chemiluminescent labeling, are further steps to confirm their potential applicability in immunodiagnostics.
Collapse
Affiliation(s)
- Milena Pieńkos
- Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland
| | - Beata Zadykowicz
- Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland
| |
Collapse
|
|
5
|
Yang B, Shi L, Tang Q, Liu W, Li B, Yang C, Jin Y. Automated study on kinetics and biosensing of glow-type luminescence reaction via digital microfluidics-chemiluminescence. LAB ON A CHIP 2023; 23:785-792. [PMID: 36723360 DOI: 10.1039/d2lc01153k] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/18/2023]
Abstract
Automated manipulation of discrete droplets by digital microfluidics (DMF) combined with chemiluminescence (CL) is promising to achieve automated and sensitive biosensing and bioanalysis. Herein, a DMF-CL device was built to automatically study CL kinetics and biosensing of a glow-type CL reaction. Copper-cysteine nanoparticles (Cu/CysNP) were synthesized as a new CL catalyst to extend the CL reaction of luminol-H2O2 to more than 10 min. The automated manipulation of droplets reduced reagent costs and manual errors, leading to real-time, automated, and reliable study of CL kinetics. The CL kinetics curve collected by the DMF-CL integration device is in accordance with that of a commercial CL analyser. The long-lasting luminescence ensured automated, sensitive, and reliable detection of H2O2 as a direct or indirect analyte of the cascade catalytic reaction. Moreover, an innovative asymmetrical splitting method is proposed to quickly and precisely generate daughter droplets to ensure uniformity of the droplets and good repeatability of the DMF-CL measurements. Therefore, the DMF-CL analysis holds great potential for achieving online and automatic analysis and biosensing.
Collapse
Affiliation(s)
- Bing Yang
- Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
| | - Lu Shi
- Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
| | - Qiaorong Tang
- Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
| | - Wei Liu
- Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
| | - Baoxin Li
- Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
| | - Chaoyong Yang
- State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
| | - Yan Jin
- Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
| |
Collapse
|
|
6
|
Dai S, Li Q, Li W, Zhang Y, Dou M, Xu R, Wang T, Lu X, Wang F, Li J. Advances in functional photonic crystal materials for the analysis of chemical hazards in food. Compr Rev Food Sci Food Saf 2022; 21:4900-4920. [PMID: 36117270 DOI: 10.1111/1541-4337.13036] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/29/2022] [Revised: 07/17/2022] [Accepted: 08/16/2022] [Indexed: 01/28/2023]
Abstract
Chemical contaminants in food generally include natural toxins (mycotoxins, animal toxins, and phytotoxins), pesticides, veterinary drugs, environmental pollutants, heavy metals, and illegal additives. Developing a low-cost, simple, and rapid detection technology for harmful substances in food is urgently needed. Analytical methods based on different advanced materials have been developed into rapid detection methods for food samples. In particular, photonic crystal (PC) materials have a unique surface periodic structure, structural color, a large surface area, easy integration with photoelectronic and magnetic devices which have great advantages in the development of rapid, low-cost, and highly sensitive analytical methods. This review focuses on the PC materials in the view of their fabrication processes, functionalized recognition components for the specific recognition of hazardous substances, and applications in the separation, enrichment, and detection of chemical hazards in real samples. Suspension array based on three-dimensional PC microspheres by droplet-based microfluidic assembly is a great promising and powerful platform for food safety detection fields. For the PCs selective analysis, biological antibodies, aptamers, and molecularly imprinted polymers (MIPs) could be modified for specific recognition of target substances, particularly MIPs because of their low-cost and easy mass production. Based on these functional PCs, various toxic and hazardous substances can be selectively enriched or recognized in real samples and further quantified in combination of liquid chromatography method or optical detection methods including fluorescence, chemiluminescence, and Raman spectroscopy.
Collapse
Affiliation(s)
- Shijie Dai
- School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China
| | - Qianjin Li
- School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China
| | - Wei Li
- Medical Imaging Center, the First Affiliated Hospital, Jinan University, Guangzhou, China
| | - Yaodan Zhang
- School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China
| | - Menghua Dou
- School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China
| | - Ruimin Xu
- School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China
| | - Tingting Wang
- School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China
| | - Xiaoyue Lu
- School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China
| | - Fenying Wang
- College of Chemistry, Nanchang University, Nanchang, China
| | - Jianlin Li
- School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China
| |
Collapse
|
|
7
|
Pieńkos M, Zadykowicz B. Solvent effect on chemiluminescence of acridinium thioester: a computational study. Chemphyschem 2022; 23:e202200166. [PMID: 35607880 DOI: 10.1002/cphc.202200166] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/11/2022] [Revised: 05/14/2022] [Indexed: 11/09/2022]
Abstract
Chemiluminescent labelling, which is one of the promising procedures of modern immunodiagnostics, is increasingly carried out using acridinium derivatives, oxidant and alkaline aqueous environment. However, the efficiency of chemiluminescence of luminol or acridinium esters is higher in non-aqueous solvents such as DMSO or acetonitrile. Therefore, the search for a new environment of chemiluminescence reaction, especially this characterized by the higher quantum yield of chemiluminescence, is one of the aims of the research undertaken. Using computational methods (DFT and TD DFT with PCM model of solvent), we examined thermodynamic and kinetic data concerning the chemiluminescence and competitive dark pathways. Our results suggest that better characteristics of chemiluminescence reaction of acridinium thioester are observed in nonpolar solvents, such as methylcyclohexane, n-hexane and n-pentane, than in aqueous media used so far. Further experimental verification is necessary to confirm the possible application of proposed nonpolar solvents in chemiluminescent labelling and hence in immunodiagnostics.
Collapse
Affiliation(s)
- Milena Pieńkos
- University of Gdansk: Uniwersytet Gdanski, Faculty of Chemistry, Wita Stwosza 63, 80-308, Gdańsk, POLAND
| | - Beata Zadykowicz
- University of Gdansk: Uniwersytet Gdanski, Faculty of Chemistry, Wita Stwosz 63, 80-308, Gdansk, POLAND
| |
Collapse
|
|
8
|
UTAGAWA Y, ITO K, INOUE KY, NASHIMOTO Y, INO K, SHIKU H. Electrochemical Substrates and Systems for Enzyme-Based Bioassays. BUNSEKI KAGAKU 2022. [DOI: 10.2116/bunsekikagaku.71.109] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
Affiliation(s)
| | - Kentaro ITO
- Graduate School of Environmental Studies, Tohoku University
| | - Kumi Y. INOUE
- Center for Basic Education, Faculty of Engineering, Graduate Faculty of Interdisciplinary Research, University of Yamanashi
| | - Yuji NASHIMOTO
- Frontier Research Institute for Interdisciplinary Sciences, Tohoku University
| | - Kosuke INO
- Graduate School of Engineering, Tohoku University
| | | |
Collapse
|
|
9
|
Degirmenci A, Sonkaya Ö, Soylukan C, Karaduman T, Algi F. BODIPY and 2,3-Dihydrophthalazine-1,4-Dione Conjugates As Heavy Atom-Free Chemiluminogenic Photosensitizers. ACS APPLIED BIO MATERIALS 2021; 4:5090-5098. [PMID: 35007057 DOI: 10.1021/acsabm.1c00328] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/08/2023]
Abstract
We disclose an interesting concept for developing heavy atom-free chemiluminogenic photosensitizers. To accomplish this, conjugates 2 and 3, which are composed of boron-dipyrromethene (BODIPY) and 2,3-dihydrophthalazine-1,4-dione units, are investigated. 2 and 3 are compared in terms of their photophysical properties, chemiluminescence responses, and singlet oxygen production. Strikingly, the results indicate that decoration of BODIPY with the 2,3-dihydrophthalazine-1,4-dione scaffold boosts the singlet oxygen generation. Furthermore, treatment of epidermoid laryngeal carcinoma Hep-2 (Hep-2) cells with conjugates 2 and 3 results in efficient cellular internalization which ensures live- cell imaging of Hep-2 cells. Finally, it is noteworthy that in vitro cytotoxicity assays reveal that both 2 and 3 induce cytotoxicity when illuminated with red light. Thus, 2 and 3 represent heavy atom-free chemiluminogenic photosensitizers.
Collapse
Affiliation(s)
- Aysun Degirmenci
- Department of Biotechnology & ASUBTAM M. Bilmez BioNanoTech Lab., Aksaray University, TR-68100 Aksaray, Turkey
| | - Ömer Sonkaya
- Department of Chemistry & ASUBTAM M. Bilmez BioNanoTech Lab., Aksaray University, TR-68100 Aksaray, Turkey
| | - Caner Soylukan
- Department of Biotechnology & ASUBTAM M. Bilmez BioNanoTech Lab., Aksaray University, TR-68100 Aksaray, Turkey
| | - Tuğçe Karaduman
- Department of Biotechnology & ASUBTAM M. Bilmez BioNanoTech Lab., Aksaray University, TR-68100 Aksaray, Turkey
| | - Fatih Algi
- Department of Biotechnology & ASUBTAM M. Bilmez BioNanoTech Lab., Aksaray University, TR-68100 Aksaray, Turkey
| |
Collapse
|
|
10
|
Affiliation(s)
- Xinping Zhang
- State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing China
| | - Xiaoyang Liu
- State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing China
| | - Yuxin Guo
- State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing China
| | - Fu‐Gen Wu
- State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering Southeast University Nanjing China
| |
Collapse
|
|
11
|
Favero V, Veríssimo CDM, Piovesan AR, Morassutti AL, Souto AA, Bittencourt HR, Pascoal VF, Lindholz CG, Jones MK, Souza RP, Rigo FDV, Carlini CR, Graeff-Teixeira C. A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method. PLoS Negl Trop Dis 2020; 14:e0008500. [PMID: 32730339 PMCID: PMC7437924 DOI: 10.1371/journal.pntd.0008500] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/21/2019] [Revised: 08/19/2020] [Accepted: 06/18/2020] [Indexed: 11/19/2022] Open
Abstract
Schistosomiasis remains a serious public health problem in tropical regions, affecting more than 250 million people. Sensitive diagnostic methods represent key tools for disease elimination, in particular in areas with low endemicity. Advances in the use of luminol-based chemiluminescent techniques have enabled greater sensitivity and speed in obtaining results in different diagnostic settings. In this study, we developed a luminol-H2O2 chemiluminescence (CL) method to detect Schistosoma mansoni eggs in human fecal sediments processed by the Helmintex (HTX) method. After S. mansoni eggs were incubated with a solution of luminol-H2O2 the light emission was detected and measured by spectrophotometry at 431 nm for 5 min, using detection and counts of eggs by bright field optical microscopy as a reference. CL intensity was found to correlate with different sources and numbers of eggs. Furthermore, our results showed that the CL method can distinguish positive from negative samples with 100% sensitivity and 71% specificity. To our knowledge, this is the first study to report the use of CL for the diagnosis of helminths from fecal samples. The combination of the HTX method with CL represents an important advance in providing a reference method with the highest standards of sensitivity. Schistosomiasis, the infection caused by parasites of the genus Schistosoma, is still a significant health problem in many countries, despite some successful control efforts. In areas where parasite load is low and few eggs are released in feces, most diagnostic tests fail to detect the infections. Helmintex is a very sensitive, but time consuming, egg-detection method, with most time being consumed in observations of samples at the microscope. Here we report standardization of an innovative luminol-H2O2 chemiluminescence reaction triggered by Schistosoma mansoni eggs that may improve their detection in feces. Improved diagnostic tests are urgently required as part of the schistosomiasis elimination efforts.
Collapse
Affiliation(s)
- Vivian Favero
- Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
- Neurotoxins Laboratory (LaNeuroTox), School of Medicine, Brain Institute and School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
- * E-mail:
| | - Carolina De Marco Veríssimo
- Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
- Centre for One Health and Ryan Institute, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland
| | - Angela R. Piovesan
- Neurotoxins Laboratory (LaNeuroTox), School of Medicine, Brain Institute and School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
- Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
| | - Alessandra L. Morassutti
- Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
| | - André A. Souto
- Polytechnic School, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
| | - Hélio R. Bittencourt
- Polytechnic School, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
| | - Vanessa F. Pascoal
- Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
| | - Catieli G. Lindholz
- Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
| | - Malcolm K. Jones
- School of Veterinary Science, The University of Queensland, Brisbane, Qld, Australia, 4072
| | - Renata P. Souza
- Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
| | - Francine De Vargas Rigo
- Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
| | - Célia R. Carlini
- Neurotoxins Laboratory (LaNeuroTox), School of Medicine, Brain Institute and School of Medicine, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
| | - Carlos Graeff-Teixeira
- Research Group on Biomedical Parasitology, School of health and life sciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil
- Infectious Diseases Unit, Center for Health Sciences, Federal University of Espirito Santo, Vitoria, Brazil
| |
Collapse
|
|
12
|
Pieńkos M, Zadykowicz B. Computational Insights on the Mechanism of the Chemiluminescence Reaction of New Group of Chemiluminogens-10-Methyl-9-thiophenoxycarbonylacridinium Cations. Int J Mol Sci 2020; 21:E4417. [PMID: 32575922 PMCID: PMC7420290 DOI: 10.3390/ijms21124417] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/01/2020] [Revised: 06/19/2020] [Accepted: 06/20/2020] [Indexed: 11/16/2022] Open
Abstract
Immunodiagnostics, in which one of the promising procedures is the chemiluminescent labelling, is essential to facilitate the detection of infections in a human organism. One of the standards commonly used in luminometric assays is luminol, which characterized by low quantum yield in aqueous environments. Acridinium esters have better characteristics in this topic. Therefore, the search for new derivatives, especially those characterized by the higher quantum yield of chemiluminescence, is one of the aims of the research undertaken. Using the proposed mechanism of chemiluminescence, we examined the effect of replacing a single atom within a center of reaction on the efficient transformation of substrates into electronically excited products. The density functional theory (DFT) and time dependent (TD) DFT calculated thermodynamic and kinetic data concerning the chemiluminescence and competitive dark pathways suggests that some of the scrutinized derivatives have better characteristics than the chemiluminogens used so far. Synthesis of these candidates for efficient chemiluminogens, followed by studies of their chemiluminescent properties, and ultimately in chemiluminescent labelling, are further steps to confirm their potential applicability in immunodiagnostics.
Collapse
Affiliation(s)
| | - Beata Zadykowicz
- Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland;
| |
Collapse
|
|
13
|
Amjadi M, Hallaj T, Manzoori JL, Shahbazsaghir T. An amplified chemiluminescence system based on Si-doped carbon dots for detection of catecholamines. SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2018; 201:223-228. [PMID: 29753967 DOI: 10.1016/j.saa.2018.04.058] [Citation(s) in RCA: 17] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/18/2017] [Revised: 04/24/2018] [Accepted: 04/29/2018] [Indexed: 06/08/2023]
Abstract
We report on a chemiluminescence (CL) system based on simultaneous enhancing effect of Si-doped carbon dots (Si-CDs) and cetyltrimethylammonium bromide (CTAB) on HCO3--H2O2 reaction. The possible CL mechanism is investigated and discussed. Excited-state Si-CDs was found to be the final emitting species, which are probably produced via electron and hole injection by oxy-radicals. The effect of several other heteroatom-doped CDs and undoped CDs was also investigated and compared with Si-CDs. Furthermore, it was found that catecholamines such as dopamine, adrenaline and noradrenaline remarkably diminish the CL intensity of Si-CD-HCO3--H2O2-CTAB system. By taking advantage of this fact, a sensitive probe was designed for determination of dopamine, adrenaline and noradrenaline with a limit of detection of 0.07, 0.60 and 0.01 μM, respectively. The method was applied to the determination of catecholamines in human plasma samples.
Collapse
Affiliation(s)
- Mohammad Amjadi
- Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz 5166616471, Iran.
| | - Tooba Hallaj
- Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz 5166616471, Iran
| | - Jamshid L Manzoori
- Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz 5166616471, Iran
| | - Tahmineh Shahbazsaghir
- Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz 5166616471, Iran
| |
Collapse
|
|
14
|
Habimana JDD, Ji J, Sun X. Minireview: Trends in Optical-Based Biosensors for Point-Of-Care Bacterial Pathogen Detection for Food Safety and Clinical Diagnostics. ANAL LETT 2018. [DOI: 10.1080/00032719.2018.1458104] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/11/2022]
Affiliation(s)
- Jean de Dieu Habimana
- State Key Laboratory of Food Science and Technology, School of Food Science, National Engineering Research Center for Functional Foods, Synergetic Innovation Center of Food Safety, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China
- Department of Food Science and Technology, School of Food Science and Technology, University of Rwanda, Kigali, Rwanda
| | - Jian Ji
- State Key Laboratory of Food Science and Technology, School of Food Science, National Engineering Research Center for Functional Foods, Synergetic Innovation Center of Food Safety, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China
| | - Xiulan Sun
- State Key Laboratory of Food Science and Technology, School of Food Science, National Engineering Research Center for Functional Foods, Synergetic Innovation Center of Food Safety, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China
| |
Collapse
|
|
15
|
Roth-Konforti ME, Bauer CR, Shabat D. Unprecedented Sensitivity in a Probe for Monitoring Cathepsin B: Chemiluminescence Microscopy Cell-Imaging of a Natively Expressed Enzyme. Angew Chem Int Ed Engl 2017. [DOI: 10.1002/ange.201709347] [Citation(s) in RCA: 54] [Impact Index Per Article: 6.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Affiliation(s)
| | | | - Doron Shabat
- School of Chemistry, Faculty of Exact Sciences; Tel Aviv University; Tel Aviv 69978 Israel
| |
Collapse
|
|
16
|
Roth-Konforti ME, Bauer CR, Shabat D. Unprecedented Sensitivity in a Probe for Monitoring Cathepsin B: Chemiluminescence Microscopy Cell-Imaging of a Natively Expressed Enzyme. Angew Chem Int Ed Engl 2017; 56:15633-15638. [DOI: 10.1002/anie.201709347] [Citation(s) in RCA: 104] [Impact Index Per Article: 13.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/10/2017] [Indexed: 12/16/2022]
Affiliation(s)
| | | | - Doron Shabat
- School of Chemistry, Faculty of Exact Sciences; Tel Aviv University; Tel Aviv 69978 Israel
| |
Collapse
|
|
17
|
Wang J, Lee TS, Zhang Z, Tung CH. A Bioluminogenic Probe for Monitoring Tyrosinase Activity. Chem Asian J 2017; 12:397-400. [PMID: 28052521 DOI: 10.1002/asia.201601659] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/06/2016] [Revised: 12/29/2016] [Indexed: 11/10/2022]
Abstract
A bioluminogenic probe based on luciferin was designed and synthesized to monitor tyrosinase activity. This probe was efficient in assessing tyrosinase activity in a buffered aqueous solution and in measuring endogenous tyrosinase activity in melanoma cells.
Collapse
Affiliation(s)
- Jianguang Wang
- Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medicine, 413 East 69th Street, New York, NY, 10065, USA.,Current address: School of Chemical and Environmental Engineering, Anyang Institute of Technology, West of HuangHe Road, Anyang, 455000, PR China
| | - Tae Sup Lee
- Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medicine, 413 East 69th Street, New York, NY, 10065, USA.,Current address: Division of RI-Convergence Research, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul, 01812, Republic of Korea
| | - Zhe Zhang
- Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medicine, 413 East 69th Street, New York, NY, 10065, USA
| | - Ching-Hsuan Tung
- Molecular Imaging Innovations Institute, Department of Radiology, Weill Cornell Medicine, 413 East 69th Street, New York, NY, 10065, USA
| |
Collapse
|
|
18
|
Holec-Gąsior L, Ferra B, Czechowska J, Serdiuk IE, Krzymiński K, Kur J. A novel chemiluminescent immunoassay for detection of Toxoplasma gondii IgG in human sera. Diagn Microbiol Infect Dis 2016; 85:422-5. [PMID: 27298047 DOI: 10.1016/j.diagmicrobio.2016.05.013] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/03/2016] [Revised: 04/28/2016] [Accepted: 05/20/2016] [Indexed: 10/21/2022]
Abstract
This study describes Toxoplasma gondii IgG chemiluminescent immunoassay (CLIA) based on the use of a novel immunochemical reagents in the form of the conjugates of original acridinium ester (AE) labels attached to antibodies and SAG2-GRA1-ROP1L chimeric antigen and shows that this test is useful for diagnostic purposes.
Collapse
Affiliation(s)
- Lucyna Holec-Gąsior
- Gdańsk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, 11/12 G. Narutowicza Str., 80-233, Gdańsk, Poland.
| | - Bartłomiej Ferra
- Gdańsk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, 11/12 G. Narutowicza Str., 80-233, Gdańsk, Poland
| | - Justyna Czechowska
- University of Gdańsk, Faculty of Chemistry, Department of Physical Chemistry, 63 Wita Stwosza Str., 80-308, Gdańsk, Poland
| | - Illia E Serdiuk
- University of Gdańsk, Faculty of Chemistry, Department of Physical Chemistry, 63 Wita Stwosza Str., 80-308, Gdańsk, Poland
| | - Karol Krzymiński
- University of Gdańsk, Faculty of Chemistry, Department of Physical Chemistry, 63 Wita Stwosza Str., 80-308, Gdańsk, Poland
| | - Józef Kur
- Gdańsk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, 11/12 G. Narutowicza Str., 80-233, Gdańsk, Poland
| |
Collapse
|
|
19
|
Yan N, Zhu Z, He D, Jin L, Zheng H, Hu S. Simultaneous Determination of Size and Quantification of Gold Nanoparticles by Direct Coupling Thin layer Chromatography with Catalyzed Luminol Chemiluminescence. Sci Rep 2016; 6:24577. [PMID: 27080702 PMCID: PMC4832333 DOI: 10.1038/srep24577] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/09/2015] [Accepted: 03/30/2016] [Indexed: 12/17/2022] Open
Abstract
The increasing use of metal-based nanoparticle products has raised concerns in particular for the aquatic environment and thus the quantification of such nanomaterials released from products should be determined to assess their environmental risks. In this study, a simple, rapid and sensitive method for the determination of size and mass concentration of gold nanoparticles (AuNPs) in aqueous suspension was established by direct coupling of thin layer chromatography (TLC) with catalyzed luminol-H2O2 chemiluminescence (CL) detection. For this purpose, a moving stage was constructed to scan the chemiluminescence signal from TLC separated AuNPs. The proposed TLC-CL method allows the quantification of differently sized AuNPs (13 nm, 41 nm and 100 nm) contained in a mixture. Various experimental parameters affecting the characterization of AuNPs, such as the concentration of H2O2, the concentration and pH of the luminol solution, and the size of the spectrometer aperture were investigated. Under optimal conditions, the detection limits for AuNP size fractions of 13 nm, 41 nm and 100 nm were 38.4 μg L(-1), 35.9 μg L(-1) and 39.6 μg L(-1), with repeatabilities (RSD, n = 7) of 7.3%, 6.9% and 8.1% respectively for 10 mg L(-1) samples. The proposed method was successfully applied to the characterization of AuNP size and concentration in aqueous test samples.
Collapse
Affiliation(s)
- Neng Yan
- State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, CN 430074, China
| | - Zhenli Zhu
- State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, CN 430074, China
| | - Dong He
- State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, CN 430074, China
| | - Lanlan Jin
- State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, CN 430074, China
| | - Hongtao Zheng
- Faculty of Material Science and Chemistry, China University of Geosciences, Wuhan, CN 430074, China
| | - Shenghong Hu
- State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, CN 430074, China
- Faculty of Earth Sciences, China University of Geosciences, Wuhan, CN 430074, China
| |
Collapse
|
|
20
|
Lee ES, Deepagan VG, You DG, Jeon J, Yi GR, Lee JY, Lee DS, Suh YD, Park JH. Nanoparticles based on quantum dots and a luminol derivative: implications for in vivo imaging of hydrogen peroxide by chemiluminescence resonance energy transfer. Chem Commun (Camb) 2016; 52:4132-5. [DOI: 10.1039/c5cc09850e] [Citation(s) in RCA: 54] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/04/2023]
Abstract
Hybrid nanoparticles allow for imaging hydrogen peroxide via chemiluminescence resonance energy transfer in the near-infrared wavelength range.
Collapse
Affiliation(s)
- Eun Sook Lee
- Department of Health Sciences and Technology
- SAIHST
- Sungkyunkwan University
- Suwon 440-746
- Republic of Korea
| | - V. G. Deepagan
- Department of Polymer Science and Engineering
- Sungkyunkwan University
- Suwon 440-746
- Republic of Korea
| | - Dong Gil You
- School of Chemical Engineering
- Sungkyunkwan University
- Suwon 440-746
- Republic of Korea
| | - Jueun Jeon
- School of Chemical Engineering
- Sungkyunkwan University
- Suwon 440-746
- Republic of Korea
| | - Gi-Ra Yi
- School of Chemical Engineering
- Sungkyunkwan University
- Suwon 440-746
- Republic of Korea
| | - Jung Young Lee
- School of Chemical Engineering
- Sungkyunkwan University
- Suwon 440-746
- Republic of Korea
| | - Doo Sung Lee
- School of Chemical Engineering
- Sungkyunkwan University
- Suwon 440-746
- Republic of Korea
| | - Yung Doug Suh
- Research Center for Convergence Nanobiotechnology (RC2NT)
- Korea Research Institute of Chemical Technology (KRICT)
- Daejeon 305-600
- Republic of Korea
- School of Chemical Engineering
| | - Jae Hyung Park
- Department of Health Sciences and Technology
- SAIHST
- Sungkyunkwan University
- Suwon 440-746
- Republic of Korea
| |
Collapse
|
|
21
|
Kim TJ, Tuerkcan S, Ceballos A, Pratx G. Modular platform for low-light microscopy. BIOMEDICAL OPTICS EXPRESS 2015; 6:4585-4598. [PMID: 26601020 PMCID: PMC4646564 DOI: 10.1364/boe.6.004585] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/17/2015] [Revised: 10/11/2015] [Accepted: 10/19/2015] [Indexed: 06/01/2023]
Abstract
Cell imaging using low-light techniques such as bioluminescence, radioluminescence, and low-excitation fluorescence has received increased attention, particularly due to broad commercialization of highly sensitive detectors. However, the dim signals are still regarded as difficult to image using conventional microscopes, where the only low-light microscope in the market is primarily optimized for bioluminescence imaging. Here, we developed a novel modular microscope that is cost-effective and suitable for imaging different low-light luminescence modes. Results show that this microscope system features excellent aberration correction capabilities and enhanced image resolution, where bioluminescence, radioluminescence and epifluorescence images were captured and compared with the commercial bioluminescence microscope.
Collapse
Affiliation(s)
- Tae Jin Kim
- Department of Radiation Oncology (Medical Physics), Stanford University, 1050 Arastradero Rd., Palo Alto, CA, 94304, USA
| | - Silvan Tuerkcan
- Department of Radiation Oncology (Medical Physics), Stanford University, 1050 Arastradero Rd., Palo Alto, CA, 94304, USA
| | - Andrew Ceballos
- Department of Electrical Engineering, Stanford University, 350 Serra Mall, Stanford, CA, 94305, USA
| | - Guillem Pratx
- Department of Radiation Oncology (Medical Physics), Stanford University, 1050 Arastradero Rd., Palo Alto, CA, 94304, USA
| |
Collapse
|
|
22
|
TOMIZAWA MINORU, SHINOZAKI FUMINOBU, FUGO KAZUNORI, MOTOYOSHI YASUFUMI, SUGIYAMA TAKAO, YAMAMOTO SHIGENORI, KISHIMOTO TAKASHI, ISHIGE NAOKI. Anti-mitochondrial M2 antibody-positive autoimmune hepatitis. Exp Ther Med 2015; 10:1419-1422. [PMID: 26622500 PMCID: PMC4578094 DOI: 10.3892/etm.2015.2694] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/05/2014] [Accepted: 08/11/2015] [Indexed: 02/07/2023] Open
Abstract
Anti-mitochondrial M2 antibody (AMA-M2) is specific to primary biliary cirrhosis (PBC), but can also be found in certain patients with autoimmune hepatitis (AIH). Effective methods of differentiating between PBC and AIH are required, as their clinical course and management are different. Titers of AMA-M2 were analyzed before and after follow-up in patients with PBC or AIH. Patients who underwent liver biopsy and were diagnosed with either AIH (10 patients) or PBC (3 patients) were enrolled in the study. The AMA-M2 antibody titers of these patients were analyzed upon hospital admission. AMA-M2 reacted with the pyruvate dehydrogenase complex-E2, branched-chain 2-oxo acid dehydrogenase complex and 2-oxoglutaric acid dehydrogenase complex in the assay utilized for this study. The cut-off value for AMA-M2 was 5. Six AIH patients were AMA-M2(-) and 4 were AMA-M2(+). The titer for the AIH patients who were AMA-M2(+) was 24.8±14.8, compared with 324±174 in the patients with PBC (P=0.0138). Three AMA-M2(+) AIH patients were followed-up after liver biopsy. The AMA-M2 levels had decreased in all 3 patients, becoming undetectable in 2 of them. In conclusion, certain patients with AIH in this study were found to be AMA-M2(+), but the titers were significantly lower than those in the patients with PBC. At follow-up, the AIH patients exhibited decreased AMA-M2 titers.
Collapse
Affiliation(s)
- MINORU TOMIZAWA
- Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan
| | - FUMINOBU SHINOZAKI
- Department of Radiology, National Hospital Organization Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan
| | - KAZUNORI FUGO
- Department of Molecular Pathology, Chiba University Graduate School of Medicine, Chiba, Chiba 260-8670, Japan
| | - YASUFUMI MOTOYOSHI
- Department of Neurology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan
| | - TAKAO SUGIYAMA
- Department of Rheumatology, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan
| | - SHIGENORI YAMAMOTO
- Department of Pediatrics, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan
| | - TAKASHI KISHIMOTO
- Department of Molecular Pathology, Chiba University Graduate School of Medicine, Chiba, Chiba 260-8670, Japan
| | - NAOKI ISHIGE
- Department of Neurosurgery, National Hospital Organization, Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan
| |
Collapse
|
|
23
|
Gonta A. Towards Screening the Enhance of Luminescence Properties of Modified Tannins in Alkaline Solution by a Fenton-Like Reaction. CHEMISTRY JOURNAL OF MOLDOVA 2015. [DOI: 10.19261/cjm.2015.10(1).15] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open
|
|
24
|
Herbáth M, Papp K, Balogh A, Matkó J, Prechl J. Exploiting fluorescence for multiplex immunoassays on protein microarrays. Methods Appl Fluoresc 2014; 2:032001. [PMID: 29148470 DOI: 10.1088/2050-6120/2/3/032001] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/19/2022]
Abstract
Protein microarray technology is becoming the method of choice for identifying protein interaction partners, detecting specific proteins, carbohydrates and lipids, or for characterizing protein interactions and serum antibodies in a massively parallel manner. Availability of the well-established instrumentation of DNA arrays and development of new fluorescent detection instruments promoted the spread of this technique. Fluorescent detection has the advantage of high sensitivity, specificity, simplicity and wide dynamic range required by most measurements. Fluorescence through specifically designed probes and an increasing variety of detection modes offers an excellent tool for such microarray platforms. Measuring for example the level of antibodies, their isotypes and/or antigen specificity simultaneously can offer more complex and comprehensive information about the investigated biological phenomenon, especially if we take into consideration that hundreds of samples can be measured in a single assay. Not only body fluids, but also cell lysates, extracted cellular components, and intact living cells can be analyzed on protein arrays for monitoring functional responses to printed samples on the surface. As a rapidly evolving area, protein microarray technology offers a great bulk of information and new depth of knowledge. These are the features that endow protein arrays with wide applicability and robust sample analyzing capability. On the whole, protein arrays are emerging new tools not just in proteomics, but glycomics, lipidomics, and are also important for immunological research. In this review we attempt to summarize the technical aspects of planar fluorescent microarray technology along with the description of its main immunological applications.
Collapse
Affiliation(s)
- Melinda Herbáth
- Department of Immunology, Eötvös Loránd University, Budapest, 1117 Hungary
| | | | | | | | | |
Collapse
|
|
25
|
Multiwell cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection: development, characterization and comparison with cooled-CCD luminograph. Anal Bioanal Chem 2014; 406:5645-56. [DOI: 10.1007/s00216-014-7971-9] [Citation(s) in RCA: 28] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/31/2014] [Revised: 05/26/2014] [Accepted: 06/12/2014] [Indexed: 11/24/2022]
|
|
26
|
Yeh HC, Lin WY. Enhancement of Chemiluminescence from the Oxidation of Luminol with Hydrogen Peroxide Catalyzed by Mn(III)-Microperoxidase 8. J CHIN CHEM SOC-TAIP 2013. [DOI: 10.1002/jccs.200300011] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022]
|
|
27
|
Yao HC, Yang XF, Li H. Sensitive Determination of Nanogram Levels of Diacerein in a Pharmaceutical Formulation by Flow Injection Chemiluminescence Analysis. J CHIN CHEM SOC-TAIP 2013. [DOI: 10.1002/jccs.200700136] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/11/2022]
|
|
28
|
Yeh HC, Hsu WT, Lin WY. Enhancement in Chemiluminescence by Carbonate for Cobalt(II)-catalyzed Oxidation of Luminol with Hydrogen Peroxide. J CHIN CHEM SOC-TAIP 2013. [DOI: 10.1002/jccs.200500093] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/06/2022]
|
|
29
|
Yang JY, Zhang Y, Lei HT, Wang H, Xu ZL, Shen YD, Sun YM. Development of an Ultra-sensitive Chemiluminescence Enzyme Immunoassay for the Determination of Diethylstilbestrol in Seafood. ANAL LETT 2013. [DOI: 10.1080/00032719.2013.798794] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/26/2022]
|
|
30
|
Alarfaj NA, El-Tohamy MF. Automated Sequential-injection Chemiluminescence Determination of Glucosamine Sulphate via Luminol-Hydrogen Peroxide System. J CHIN CHEM SOC-TAIP 2013. [DOI: 10.1002/jccs.201300187] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022]
|
|
31
|
|
|
32
|
|
|
33
|
Richard JA, Jean L, Schenkels C, Massonneau M, Romieu A, Renard PY. Self-cleavable chemiluminescent probes suitable for protease sensing. Org Biomol Chem 2009; 7:2941-57. [DOI: 10.1039/b905725k] [Citation(s) in RCA: 36] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
|
|
34
|
|
|
35
|
|
|
36
|
Detecting AhR ligands in sediments using bioluminescent reporter yeast. Biosens Bioelectron 2008; 23:1850-5. [DOI: 10.1016/j.bios.2008.02.026] [Citation(s) in RCA: 16] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/06/2007] [Revised: 02/27/2008] [Accepted: 02/28/2008] [Indexed: 11/24/2022]
|
|
37
|
Gutiérrez AM, Reboredo GR, Mosca SM, Catalá A. An allometric study of fatty acids and sensitivity to lipid peroxidation of brain microsomes and mitochondria isolated from different bird species. Comp Biochem Physiol A Mol Integr Physiol 2008; 150:359-65. [PMID: 18508398 DOI: 10.1016/j.cbpa.2008.04.597] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/26/2008] [Revised: 04/16/2008] [Accepted: 04/16/2008] [Indexed: 11/26/2022]
Abstract
The objective of this investigation was to examine the relationship between body size, fatty acid composition and sensitivity to lipid peroxidation of mitochondria and microsomes isolated from the brain of different size bird species: manon, quail, pigeon, duck and goose, representing a 372-fold range of body mass. Fatty acids of total lipids were determined using gas chromatography and lipid peroxidation was evaluated using a chemiluminescence assay. The allometric study of the fatty acids present in brain mitochondria and microsomes of the different bird species showed a small number of significant allometric trends. In mitochondria the percentage of monounsaturated fatty acids, was significantly lower in the larger birds (r=-0.965; P<0.008). The significant allometric increase in 18:2 n-6; linoleic acid (r=0.986; P<0.0143), polyunsaturated (r=0.993; P<0.007) and total unsaturated (r=0.966; P<0.034) in brain microsomes but not in mitochondria may indicate a preferential incorporation of this fatty acid in the brain endoplasmic reticulum of the larger bird species. The brain of all birds studied had a high content of docosahexaenoic acid. However brain mitochondria but not microsomes isolated from all the birds analyzed showed a significant decrease of arachidonic and docosahexaenoic acids during lipid peroxidation. The allometric analyses of chemiluminescence were not statistically significant. In conclusion our results show absence of correlation between the sensitivity to lipid peroxidation of brain mitochondria and microsomes with body size and maximum life span.
Collapse
Affiliation(s)
- A M Gutiérrez
- Cátedra de Fisiología Animal, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, CONICET, La Plata, Argentina
| | | | | | | |
Collapse
|
|
38
|
Bacart J, Corbel C, Jockers R, Bach S, Couturier C. The BRET technology and its application to screening assays. Biotechnol J 2008; 3:311-24. [DOI: 10.1002/biot.200700222] [Citation(s) in RCA: 134] [Impact Index Per Article: 7.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022]
|
|
39
|
Feroci G, Roda A, Fini A. Study of the interaction between oxygen and bile salts. Bioelectrochemistry 2007; 70:524-31. [PMID: 17289441 DOI: 10.1016/j.bioelechem.2006.12.002] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/19/2006] [Revised: 11/10/2006] [Accepted: 12/15/2006] [Indexed: 11/22/2022]
Abstract
The interaction between molecular oxygen and bile salts, previously observed using chemiluminescence techniques, is studied in this paper by electrochemical techniques to further highlight the nature of the interaction. A shift of half-wave potential of the first polarographic wave for the reduction of molecular oxygen was observed in solutions in the presence of bile salts. The shift could be related to different phenomena, such as adsorption of bile salt molecules on the mercury electrode, irreversibility of the oxygen reduction reaction, pH of the solution. Experimental results suggest the exclusion of the above mentioned processes and outline the occurrence of a direct interaction between oxygen and bile salts, where the hydrophobic face of bile salt monomers and/or small aggregates are involved, enhancing so dismutation of superoxide ion produced at the electrode. The presence of bile salts in solutions containing triphenylphosphine oxide, a hydrophobic surfactant, increases also the wave of reduction of molecular oxygen. As a consequence bile salts, beside the well-assessed physiological roles, can behave as oxygen carrier and as antioxidant, preventing the oxidation of biological compounds by superoxide ion.
Collapse
|
|
40
|
Flow Injection Technique for Biochemical Analysis with Chemiluminescence Detection in Acidic Media. SENSORS 2007. [DOI: 10.3390/s7040448] [Citation(s) in RCA: 11] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
|
|
41
|
Abstract
Bioluminescent methods are gaining more and more attention among scientists due to their sensitivity, selectivity and simplicity; coupled with the fact that the bioluminescence can be monitored both in vitro and in vivo. Since the discovery of bioluminescence in the 19th century, enzymes involved in the bioluminescent process have been isolated and cloned. The bioluminescent reactions in several different organisms have also been fully characterized and used as reporters in a wide variety of biochemical assays. From the 1990s it became clear that bioluminescence can be detected and quantified directly from inside a living cell. This gave rise to numerous possibilities for the in vivo monitoring of intracellular processes non-invasively using bioluminescent molecules as reporters. This review describes recent developments in the area of bioluminescent imaging for cell biology. Newly developed imaging methods allow transcriptional/translational regulation, signal transduction, protein-protein interaction, oncogenic transformation, cell and protein trafficking, and target drug action to be monitored in vivo in real-time with high temporal and spatial resolution; thus providing researchers with priceless information on cellular functions. Advantages and limitations of these novel bioluminescent methods are discussed and possible future developments identified.
Collapse
Affiliation(s)
- Lubov Y. Brovko
- Canadian Research Institute for Food Safety and Adjunct, Food Science Department, University of Guelph, Canada
| | | |
Collapse
|
|
42
|
Gavazza MB, Catalá A. The effect of alpha-tocopherol on lipid peroxidation of microsomes and mitochondria from rat testis. Prostaglandins Leukot Essent Fatty Acids 2006; 74:247-54. [PMID: 16549341 DOI: 10.1016/j.plefa.2006.01.007] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/19/2005] [Revised: 01/08/2006] [Accepted: 01/29/2006] [Indexed: 10/24/2022]
Abstract
The testis is a remarkably active metabolic organ; hence it is suitable not only for studies of lipid metabolism in the organ itself but also for the study of lipid peroxidation processes in general. The content of fatty acids in testis is high with a prevalence of polyunsaturated fatty acids (PUFA) which renders this tissue very susceptible to lipid peroxidation. Studies were carried out to evaluate the effect of alpha-tocopherol in vitro on ascorbate-Fe(++) lipid peroxidation of rat testis microsomes and mitochondria. Chemiluminescence and fatty acid composition were used as an index of the oxidative destruction of lipids. Special attention was paid to the changes produced on the highly PUFA [C20:4 n6] and [C22:5 n6]. Lipid peroxidation of testis microsomes or mitochondria induced a significant decrease of both fatty acids. Total chemiluminescence was similar in both kinds of organelles when the peroxidized without (control) and with ascorbate-Fe(++) (peroxidized) groups were compared. Arachidonic acid was protected more efficiently than docosapentaenoic acid at all alpha-tocopherol concentrations tested when rat testis microsomes or mitochondria were incubated with ascorbate-Fe(++). The maximal percentage of inhibition in both organelles was approximately 70%; corresponding to an alpha-tocopherol concentration between 1 and 0.25 mM. IC50 values from the inhibition of alpha-tocopherol on the chemiluminescence were higher in microsomes (0.144 mM) than mitochondria (0.078 mM). The protective effect observed by alpha-tocopherol in rat testis mitochondria was higher compared with microsomes, associated with the higher amount of [C20:4 n6]+[C22:5 n6] in microsomes that in mitochondria. It is proposed that the vulnerability to lipid peroxidation of rat testis microsomes and mitochondria is different because of the different proportion of PUFA in these organelles The peroxidizability index (PI) was positively correlated with the level of long chain fatty acids. The results demonstrated the protective effect of alpha-tocopherol on lipid peroxidation in microsomes and mitochondria from rat testis.
Collapse
Affiliation(s)
- M B Gavazza
- Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Argentina
| | | |
Collapse
|
|
43
|
Haghighi B, Dadashvand R. Flow injection chemiluminescence analysis of phenolic compounds using the NCS-luminol system. Anal Bioanal Chem 2006; 384:1246-53. [PMID: 16485089 DOI: 10.1007/s00216-005-0267-3] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/10/2005] [Revised: 12/01/2005] [Accepted: 12/04/2005] [Indexed: 10/25/2022]
Abstract
A flow injection system coupled with two simple and sensitive chemiluminescence (CL) methods is described for the determination of some phenolic compounds. The methods are based on the inhibition effects of the investigated phenols on the CL signal intensities of N-chlorosuccinimide-KI-luminol (NCS-KI-luminol) and NCS-luminol systems. The influences of the chemical and hydrodynamic parameters on the decrease in CL signal intensities of NCS-KI-luminol and NCS-luminol systems for hydroquinone, catechol, and resorcinol, serving as the model compounds of analyte, were studied in the flow injection mode of analysis. Under the selected conditions, the proposed CL systems were used for the determination of some phenolic compound and analytical characteristics of the systems including calibration equation, correlation coefficient, linear dynamic range, limit of detection, and sample throughput. The limits of detection for hydroquinone, catechol, and resorcinol were 0.002, 0.01, and 0.3 microM using the NCS-KI-luminol system; for the NCS-luminol system these were 0.01, 0.17, and 1.6 microM, respectively. The relative standard deviation for 10 repeated measurements of 0.04, 0.06, and 1 microM of hydroquinone, catechol, and resorcinol were 1.9, 1.4, and 2.0%, respectively, with the NCS-KI-luminol system; for 0.2, 0.5, and 4 microM of hydroquinone, catechol, and resorcinol these were 2.6, 2.2, and 3.7%, respectively, using the NCS-luminol system. The method was applied to the determination of catechol in known environmental water samples with a relative error of less than 6%. A possible reaction mechanism of the proposed CL system is discussed briefly.
Collapse
Affiliation(s)
- Behzad Haghighi
- Department of Chemistry, Institute for Advanced Studies in Basic Sciences, 45195-1159 Zanjan, Iran.
| | | |
Collapse
|
|
44
|
Kumaresan PR, Lam KS. Screening chemical microarrays: methods and applications. MOLECULAR BIOSYSTEMS 2006; 2:259-70. [PMID: 16880944 DOI: 10.1039/b602004f] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/29/2022]
Affiliation(s)
- Pappanaicken R Kumaresan
- Division of Hematology & Oncology, Department of Internal Medicine, UC Davis Cancer Center, University of California Davis, 4501 X Street, Sacramento, CA 95817, USA
| | | |
Collapse
|
|
45
|
Gámiz-Gracia L, Garcı́a-Campaña AM, Soto-Chinchilla JJ, Huertas-Pérez JF, González-Casado A. Analysis of pesticides by chemiluminescence detection in the liquid phase. Trends Analyt Chem 2005. [DOI: 10.1016/j.trac.2005.05.009] [Citation(s) in RCA: 69] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/25/2022]
|
|
46
|
Hać S, Dobosz M, Kaczor JJ, Rzepko R, Aleksandrowicz-Wrona E, Wajda Z, Sledziński Z, Krajewski J. Neutrophil engagement and septic challenge in acute experimental pancreatitis in rats. World J Gastroenterol 2005; 11:6459-65. [PMID: 16425416 PMCID: PMC4355786 DOI: 10.3748/wjg.v11.i41.6459] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/06/2023] Open
Abstract
AIM: To investigate the influence of neutrophil adhesion molecule blockade with monoclonal antibody (MoAb CD11b) and E. coli lipopolysaccharide (LPS) administration on experimental acute pancreatitis (AP).
METHODS: AP was induced by four ip injections of cerulein (Cn) at 1-h intervals. MoAb CD 11b and LPS were administered at the beginning of the experiment.
RESULTS: The neutrophil count and chemiluminescence were diminished at the beginning of AP. The oxidative stress parameters were found within the pancreatic gland. MoAb CD 11b used for AP resulted in a significant reduction of pancreatic infiltration and pancreatitis oxidative stress parameters. Serum interleukin-6 (IL-6) was not detected in AP animals, whereas high serum IL-6 concentration was noted only in animals receiving LPS.
CONCLUSION: Neutrophils are involved in pancreatic damage in the early stage of AP. Neutrophil infiltration reduction protects the pancreatic gland from destruction during AP. LPS does not change the early course of Cn pancreatitis in rats.
Collapse
Affiliation(s)
- Stanisław Hać
- Department of General Endocrine and Transplant Surgery, Medical University of Gdańsk, Poland.
| | | | | | | | | | | | | | | |
Collapse
|
|
47
|
Mei Y, Ran L, Dongyan H, Yuying G. A novel flow-injection chemiluminescence system for the determination of guanine. LUMINESCENCE 2005; 20:307-10. [PMID: 16134213 DOI: 10.1002/bio.844] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/06/2022]
Abstract
A novel flow-injection chemiluminescence (CL) method for the determination of guanine was developed. The procedure is based on the CL reaction of guanine with hydrogen peroxide in borax buffer (pH 8.5) with Co2+ as a catalyst. The calibration graph is linear within the range of 3 x 10(-7)-9 x 10(-5) g/mL. A detection limit of 1 x 10(-7) g/mL, along with a relative standard deviation of 2.23% (3 x 10(-7) g/mL guanine, n = 11), were obtained. The present procedure was applied to the measurement of guanine in urine with recoveries of 97.5-107.5%. A possible CL mechanism of the reaction system is proposed.
Collapse
Affiliation(s)
- Yang Mei
- Department of Chemistry, Liaoning Normal University, Dalian 116029, People's Republic of China.
| | | | | | | |
Collapse
|
|
48
|
Davidsson R, Genin F, Bengtsson M, Laurell T, Emnéus J. Microfluidic biosensing systems. Part I. Development and optimisation of enzymatic chemiluminescent micro-biosensors based on silicon microchips. LAB ON A CHIP 2004; 4:481-7. [PMID: 15472732 DOI: 10.1039/b400894d] [Citation(s) in RCA: 20] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/12/2023]
Abstract
Chemiluminescent (CL) enzyme-based flow-through microchip biosensors (micro-biosensors) for detection of glucose and ethanol were developed for the purpose of monitoring real-time production and release of glucose and ethanol from microchip immobilised yeast cells. Part I of this study focuses on the development and optimisation of the micro-biosensors in a microfluidic sequential injection analysis (microSIA) system. Glucose oxidase (GOX) or alcohol oxidase (AOX) was co-immobilised with horseradish peroxidase (HRP) on porous silicon flow through microchips. The hydrogen peroxide produced from oxidation of the corresponding analyte (glucose or ethanol) took part in the chemiluminescent (CL) oxidation of luminol catalysed by HRP enhanced by addition of p-iodophenol (PIP). All steps in the microSIA system, including control of syringe pump, multiposition valve (MPV) and data readout, were computer controlled. The influence of flow rate and luminol- and PIP concentration were investigated using a 2(3)-factor experiment using the GOX-HRP sensor. It was found that all estimated single factors and the highest order of interaction were significant. The optimum was found at 250 microM luminol and 150 microM PIP at a flow rate of 18 microl min(-1), the latter as a compromise between signal intensity and analysis time. Using the optimised system settings one sample was processed within 5 min. Two different immobilisation chemistries were investigated for both micro-biosensors based on 3-aminopropyltriethoxsilane (APTS)- or polyethylenimine (PEI) functionalisation followed by glutaraldehyde (GA) activation. GOX-HRP micro-biosensors responded linear in a log-log format within the range 10-1000 microM glucose. Both had an operational stability of at least 8 days, but the PEI-GOX-HRP sensor was more sensitive. The AOX-HRP micro-biosensors responded linear (log-log) in the range between 1 and 10 mM ethanol, but the PEI-AOX-HRP sensor was in general more sensitive. Both sensors had an operational stability of at least 8 h, but with a half-life of 2-3 days.
Collapse
Affiliation(s)
- Richard Davidsson
- Department of Analytical Chemistry, Lund University, P.O. Box 124, SE-22200 Lund, Sweden
| | | | | | | | | |
Collapse
|
|
49
|
Gavazza MB, Català A. Protective effect of N-acetyl-serotonin on the nonenzymatic lipid peroxidation in rat testicular microsomes and mitochondria. J Pineal Res 2004; 37:153-60. [PMID: 15357659 DOI: 10.1111/j.1600-079x.2004.00150.x] [Citation(s) in RCA: 15] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
Abstract
N-acetyl-serotonin, the immediate precursor of melatonin in the tryptophan metabolic pathway in the pineal gland, has been reported to be an antioxidant. The aim of this study was to test the in vitro protective effect of N-acetyl-serotonin on the ascorbate-Fe(++) induced lipid peroxidation of polyunsaturated fatty acids (PUFAs) located in testis microsomes and mitochondria. We assayed increasing concentrations (0-10 mM) of N-acetyl-serotonin in testis microsomes and (0-1 mM) of N-acetyl-serotonin in testis mitochondria. Control experiments were performed by incubating microsomal and mitochondrial membranes with N-acetyl-serotonin in the absence of lipid peroxidation-inducing drugs. Special attention was paid to the changes produced on the highly PUFAs C20:4 n6 and C22:5 n6. The light emission (chemiluminescence) used as a marker of lipid peroxidation was similar in both organelles when the control and peroxidized groups were compared. N-acetyl-serotonin reduced lipid peroxidation in testicular microsomes or mitochondria for both C20:4 n6 and C22:5 n6. Both long chain PUFAs were protected when N-acetyl-serotonin was incorporated either into microsomes or mitochondria. The N-acetyl-serotonin concentration required to inhibit by approximately 70% lipid peroxidation process was 10 mM in microsomes and between 0.50 and 1 mM in mitochondria. IC 50 values calculated from the inhibition curve of N-acetyl-serotonin on the chemiluminescence rates were higher in microsomes (4.50 mM) than in mitochondria (0.25 mM). In these experimental conditions, N-acetyl-serotonin was about 18 times more potent in testicular mitochondria in inhibiting the oxidative processes than it was in testicular microsomes. These results suggest that the protective role of N-acetyl-serotonin in preserving the long PUFAs may be related to its ability to reduce lipid peroxidation.
Collapse
Affiliation(s)
- Mariana B Gavazza
- Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, B1900 AVW La Plata, Argentina
| | | |
Collapse
|
|
50
|
Hać S, Dobosz M, Kaczor J, Rzepko R. Influence of molecule CD 11b blockade on the course of acute ceruleine pancreatitis in rats. Exp Mol Pathol 2004; 77:57-65. [PMID: 15215051 DOI: 10.1016/j.yexmp.2003.12.006] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/05/2003] [Indexed: 02/07/2023]
Abstract
Polymorphonuclear cells (PMN) activation is an essential step in acute pancreatitis (AP). We investigated the activation status of PMN, oxidative stress and pancreatic damage in early stage of experimental ceruleine pancreatitis in rats. The PMN action was modulated by monoclonal antibody CD 11b administration. The circulating WBC and polymorphonuclear cells count was reduced after AP induction. Chemiluminescence of whole blood PMN was remarkably reduced in AP group and increased after MoAb CD 11b administration. The CD 11b blockade significantly reduced the WBC infiltration and malondialdehyde (MDA) concentration within pancreatic gland. These data suggest that activated PMN are an important factor in early AP pathogenesis. Neutrophil aggregation within pancreatic gland modulated by monoclonal antibody CD11b contribute to the extent of injury during the early stage of ceruleine experimental pancreatitis in rats.
Collapse
Affiliation(s)
- Stanisław Hać
- Department of General Gastroenterological and Endocrine Surgery, Medical University of Gdañsk, Gdansk, Poland.
| | | | | | | |
Collapse
|