New categorization of human vascular endothelial cells by pro- vs anti-proliferative phenotypes

Figure 1 Phenotype determination of primary cultured human vascular endothelial cells. A and B: Illustrations of the method to calculate proliferation indexes of VEC-co-cultured VSMC via ModFit LT™ software-based analyses; C: Results of flow cytometric analyses of HUVEC-co-cultured VSMC with ModFit LT™ analyses; D: Results in C were statistically evaluated (n = 3); E: Fluorescent microscopy of PKH-26-stained VSMC subjected to contact co-culture with HUVEC; F: Results of VSMC-co-culture experiments using HAEC, HCAEC and HMVEC (n = 3). VEC: Vascular endothelial cells; HAEC: Human umbilical artery endothelial cells; HCAEC: Human coronary artery endothelial cells; HUVEC: Human umbilical vein endothelial cells; HMVEC: Human microvascular endothelial cell; VSMC: Vascular smooth muscle cell.