Role of nestin in glioma invasion

Figure 4 Effect of nestin knockdown on proliferation and the expression of stem cell and differentiation markers. A: HSR-GBM1 and HSR040622 cells stably expressing the lentivirus driven nestin shRNA show no significant difference in cellular proliferation over a period of 6 d as compared with shC (scrambled control) infected cells; B: In HSR-GBM1, lowered nestin levels has no significant effect on mRNA levels of the neural stem-cell markers CD133 and OLIG2, while statistical significant reduction of mRNA levels of GFAP (glial differentiation), MAP2 (neuronal differentiation), and the intermediate filament Vimentin was observed. The expression of the L-transcript variant of the intermediate filament synemin (syn) was significantly increased; C: In HSR040622, reduced nestin levels had no effect on expression of any of the tested genes mentioned above. It is important to note that the expression of the intermediate filament Synemin was barely detected. (NS = not significant, ^aP < 0.05, ^bP < 0.01, ^dP < 0.001; all two sided t test ). shC: Scrambled control; shN: ShNestin.