Copyright
©The Author(s) 2015.
World J Transl Med. Dec 12, 2015; 4(3): 78-87
Published online Dec 12, 2015. doi: 10.5528/wjtm.v4.i3.78
Published online Dec 12, 2015. doi: 10.5528/wjtm.v4.i3.78
Figure 3 Nestin expression knockdown has no effect on glioblastoma cell proliferation.
Quantitative real time PCR and Western Blot analyses of Nestin mRNA (A) and protein (B) expression in HSR-GBM1 and HSR040622 neurospheres confirming stable knockdown of nestin expression by short hairpin RNA. HSR-GBM1 (C1, C2) and HSR040622 (C3, C4) immuno-staining confirms nestin protein reduction in shNestin (shN) infected cells (C2, C4). Quantification of Ki67 immunoreactivity shown in D (bP < 0.01, two-sided t tests). shC: Scrambled control.
- Citation: Lin A, Marchionni L, Sosnowski J, Berman D, Eberhart CG, Bar EE. Role of nestin in glioma invasion. World J Transl Med 2015; 4(3): 78-87
- URL: https://www.wjgnet.com/2220-6132/full/v4/i3/78.htm
- DOI: https://dx.doi.org/10.5528/wjtm.v4.i3.78