Antioxidant enzyme profile of two clinical isolates of Entamoeba histolytica varying in sensitivity to antiamoebic drugs

Table 2 Representative minimum inhibitory concentration plate tests of clinical isolates of Entamoeba histolytica to antiamoebic drugs

Concentration	15 h			24 h			48 h
	W-1	W-2	W-3	W-1	W-2	W-3	W-1	W-2	W-3
MIC of Entamoeba histolytica clinical isolate 980 to auranofin = 3 μmol/L
Control	+++	++++	+++	+++	+++	+++	+++	++	++
DMSO control	+++	+++	++++	+++	+++	+++	+++	++	++
1 μmol/L	++	+++	++	++	++	++	++	++	++
2 μmol/L	+	++	++	++	++	++	++	+	+
3 μmol/L	+	+	+	+	+	-	+	-	+
4 μmol/L	+	+	+	-	-	-	-	-	-
MIC of of Entamoeba histolytica clinical isolate 980 to metronidazole = 80 μmol/L
Control	+++	++++	+++	+++	+++	+++	+++	++	++
DMSO control	+++	+++	++++	+++	+++	+++	+++	++	++
50 μmol/L	++	+++	+++	++	++	++	++	++	++
60 μmol/L	++	++	++	++	++	++	++	++	+
70 μmol/L	+	++	++	++	++	++	++	+	+
80 μmol/L	++	+	++	++	+	+	+	+	-
90 μmol/L	+	+	+	+	-	-	-	-	-
MIC of Entamoeba histolytica clinical isolate 989 to auranofin = 1 μmol/L (MIC determined to be 2 μmol/L)
Control	+++	++++	+++	+++	+++	+++	+++	++	++
DMSO control	+++	+++	+++	+++	+++	+++	+++	++	++
1 μmol/L	++	+++	+++	++	+	+	+	+	+
2 μmol/L	++	++	+	+	+	+	+	-	-
3 μmol/L	+	+	+	-	-	-	-	-	-
4 μmol/L	-	-	-	-	-	-	-	-	-
MIC of of Entamoeba histolytica clinical isolate 989 to metronidazole = 30 μmol/L
Control	+++	++++	+++	+++	+++	+++	+++	++	++
DMSO control	+++	+++	+++	+++	+++	+++	+++	++	++
10 μmol/L	++	+++	+++	++	++	++	++	++	++
20 μmol/L	++	+++	++	+	++	++	++	++	+
30 μmol/L	++	++	+	+	+	+	+	+	-
40 μmol/L	+	+	+	-	-	-	-	-	-

The lowest concentration giving a + score, after 48 h, was the minimum inhibitory concentration. W-1, W-2, and W-3, represent the triplicate wells. Scores are ++++: Confluent well, covered with live motile cells; +++: 50%-70% almost confluent well filled with live motile cells; ++: 30%-50% well coverage, few cells motile); +: ≤ 20% well coverage with rounded cells; -: Dead and disintergrated cells.