Inhibition of rheumatoid arthritis by blocking connective tissue growth factor

Figure 2 Tumor necrosis factor-α positively regulated connective tissue growth factor production in synovial fibroblasts and negatively regulated connective tissue growth factor production in chondrocytes. Connective tissue growth factor (CTGF) production from the human synovial fibroblasts cell line (MH7A) and the human chondrocytes cell line (OUMS-27) stimulated with/without tumor necrosis factor (TNF)-α were evaluated by immunoblotting and quantitative real time polymerase chain reaction (PCR). TNF-α promoted CTGF production by synovial fibroblasts and inhibited the production by chondrocytes. Statistical analysis (paired t test) was performed, and ^aP < 0.05 were considered to be statistically significant. ^aP < 0.05, TNF-αvs no treat.