miR-365 promotes HOXA9-mediated differentiation of mesenchymal stem cells to nucleus pulposus cells by interacting with HIF-1α

Figure 4 miR-365 regulates differentiation of mesenchymal stem cells into nucleus pulposus cells through HOXA9/HIF-1α axis. A: Determination of proliferation ability of mesenchymal stem cells (MSCs) with indicated treatment (n = 4 per group); B: Apoptotic MSCs detected by annexin V-FITC/PI staining (n = 3 per group); C: GAGs of MSCs were determined in MSC and nucleus pulposus cell (NPC) co-culture system (n = 4 per group); D-F: The relative mRNA level of Hif-1α (D), Sox9 (E), and Kdm6a (F) in MSCs were determined in MSC and NPC co-culture system (n = 3 per group); G: The indicated protein levels in MSCs were determined by Western blot. Data are presented as the mean ± SD. ^aP < 0.001, ^bP < 0.01, and ^cP < 0.05.