miR-365 promotes HOXA9-mediated differentiation of mesenchymal stem cells to nucleus pulposus cells by interacting with HIF-1α

Figure 2 The expression level of miR-365 is increased in the spinal cord of spinal cord injury rats. A: Relative expression level of miR-365 in the spinal cord of spinal cord injury (SCI) rats (n = 3 per group); B: Relative protein level of aggrecan and type 2 collagen in the spinal cord of SCI rats; C-F: Relative mRNA level of Sox9 (C), Kdm6aA (D), Hoxa9 (E), and Hif-1α (F) in the spinal cord of SCI rats (n = 3 per group); G: Protein level of HOXA9 and HIF-1α in the spinal cord of SCI rats. Data are presented as the mean ± SD. ^aP < 0.001, ^bP < 0.01, and ^cP < 0.05 compared to the sham group.