Benefits of Ilizarov automated bone distraction for nerves and articular cartilage in experimental leg lengthening

Figure 1 Peroneal nerve, F30, M-group. A: Perineurium, fragment of transverse semithin section, methylene blue - basic fuchsin stain; B: Proliferating cells in perineurium; C: Proliferating cells in endoneurium; fragments of paraffin-embedded sections stained using antibodies to Ki-67. Magnification 1250 ×.

Figure 2 Fragments of canine Pn semithin sections. WA30, М-group. Toluidine blue stain. A: Normal condition of artery in epineurium; B: Epineural artery with closed lumen. Magnification 1250 ×.

Figure 3 Fragments of canine Tn semithin sections, WA30. A: Some large myelinated nerve fibres has visibly thinned axons and thickened myelin sheaths, М-group, methylene blue - basic fuchsin stain, magnification 1250 ×; B: Two large nerve fibers (in the lower part of the image) are hypomyelinated; А-group, toluidine blue stain, magnification 500 ×.

Figure 4 Superficial zone of articular cartilage at D28 in semithin sections. A: М-group; B: A-group. Methylene blue - basic fuchsin stain. Magnification 500 ×.

Figure 5 Chondrocytes of intermediate cartilage zone at F30. А: In the left lacuna there are two degenerated chondrocytes, in the right lacuna - chondrocyte shrinkage, chromatin condensed on periphery of caryolemma, М-group; B: Chondrocyte with well-developed functionally active vacuolated cytoplasm occupies the whole lacuna. A-group. Semithin sections. Methylene blue - basic fuchsin stain. Magnification 1250 ×.

Figure 6 Chondrocytes in SEM at F30. А: Cellular shrinkage. М-group; B: Rounded cell. A-group. Magnification 5500.