Published online May 6, 2017. doi: 10.4292/wjgpt.v8.i2.120
Peer-review started: December 2, 2016
First decision: January 5, 2017
Revised: January 25, 2017
Accepted: March 12, 2017
Article in press: March 14, 2017
Published online: May 6, 2017
To determine dynamic thiol/disulphide homeostasis in celiac disease and to examine the associate with celiac autoantibodies and gluten-free diet.
Seventy three patients with celiac disease and 73 healthy volunteers were enrolled in the study. In both groups, thiol/disulphide homeostasis was examined with a new colorimetric method recently developed by Erel and Neselioglu.
In patients with celiac disease, native thiol (P = 0.027) and total thiol (P = 0.031) levels were lower, while disulphide (P < 0.001) level, disulphide/native thiol (P < 0.001) and disulphide/total thiol (P < 0.001) ratios were higher compared to the control group. In patients who do not comply with a gluten-free diet, disulphide/native thiol ratio was found higher compared to the patients who comply with the diet (P < 0.001). In patients with any autoantibody-positive, disulphide/native thiol ratio was observed higher compared to the patients with autoantibody-negative (P < 0.05). It is found that there is a negative correlation between celiac autoantibodies, and native thiol, total thiol levels and native thiol/total thiol ratio, while a positive correlation is observed between disulphide, disulphide/native thiol and disulphide/total thiol levels.
This study is first in the literature which found that the patients with celiac disease the dynamic thiol/disulphide balance shifts through disulphide form compared to the control group.
Core tip: To the best of our knowledge, for the first time in this study, total and native thiol levels in celiac patients were found lower compared to the control group while disulphide level, disulphide/total thiol and disulphide/native thiol ratios were found to be higher. Also, this study is first in which a negative correlation between celiac autoantibodies and native thiol, and total thiol levels and native thiol/total thiol ratio is observed while there is a positive correlation between disulphide level and disulphide/native thiol and disulphide/total thiol ratios.