Differential expression of pancreatic protein and chemosensing receptor mRNAs in NKCC1-null intestine

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World Journal of Gastrointestinal Pathophysiology

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2150-5330

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Gastrointest Pathophysiol. Feb 15, 2016; 7(1): 138-149
Published online Feb 15, 2016. doi: 10.4291/wjgp.v7.i1.138

Table 1 Primer sequences used for quantitative real-time polymerase chain reaction and northern blot analyses

Name	Sequence
Pdx1	F: ACCAAAGCTCACGCGTGGAAA
	R: TGATGTGTCTCTCGGTCAAGTT
Insulin 1	F: TTGCCCTCTGGGAGCCCAAA
	R: CAGATGCTGGTGCAGCACTG
Insulin 2	F: TCTTCCTCTGGGAGTCCCAC
	R: CAGATGCTGGTGCAGCACTG
Glut2	F: CATTGCTGGAAGAAGCGTATCAG
	R: GAGACCTTCTGCTCAGTCGACG
Amylase	F: TTAACGATAATAAATGTAATGGAGAA
	R: CCCTGCTACTCCAATGTCAA
Trypsin	F: TGAGCAGTTTGTCAATTCTGCC
	R: GCATGATGTCGTTGTTCAGGG
Elastase	F: GTAGTTGCAGCCCAGAGAGG
	R: TCTGCTATGTCACAGGCTGG
Gp2	F: TCCCTGCCAGAATCACACGGT
	R: GGAGGTCCTGCCTACAGACACA
L32	F: GATCTAGCGGCCGCCATCTGTTTACGGCATCATG
	R: TAGATCGCGGCCGCCGCTCCCATAACCGATGTTGG

Forward and reverse primers for quantitative real-time polymerase chain reaction and northern blot probes are listed 5’ to 3’.

Citation: Bradford EM, Vairamani K, Shull GE. Differential expression of pancreatic protein and chemosensing receptor mRNAs in NKCC1-null intestine. World J Gastrointest Pathophysiol 2016; 7(1): 138-149
URL: https://www.wjgnet.com/2150-5330/full/v7/i1/138.htm
DOI: https://dx.doi.org/10.4291/wjgp.v7.i1.138

Bradford EM, Vairamani K, Shull GE. Differential expression of pancreatic protein and chemosensing receptor mRNAs in NKCC1-null intestine. World J Gastrointest Pathophysiol 2016; 7(1): 138-149 [PMID: 26909237 DOI: 10.4291/wjgp.v7.i1.138]

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