Prognostic significance of tumor budding, desmoplastic reaction, and lymphocytic infiltration in patients with gastric adenocarcinoma

Table 4 Tumor budding, desmoplastic reaction, and tumor-infiltrating lymphocytes in relation to clinicopathological parameters in the intestinal group

Parameters	n	PTB1 (%)	PTB2 (%)	PTB3 (%)	ITB1 (%)	ITB2 (%)	ITB3 (%)	DR1 (%)	DR2 (%)	DR3 (%)	TILs (%)	TILs (%)
Age, yr		24	39	37	30	21	49	47	28	25	52	48
< 62.14 ± 12.00	47	10 (41.7)	24 (61.5)	13 (35.1)	14 (46.7)	11 (52.4)	22 (44.9)	21 (44.7)	15 (53.6)	11 (44.0)	25 (48.1)	22 (45.8)
≥ 62.14 ± 12.00	53	14 (58.3)	15 (38.5)	24 (63.9)	16 (53.3)	10 (47.6)	27 (55.1)	26 (55.3)	13 (46.4)	14 (56.0)	27 (51.9)	26 (54.2)
Gender
Male	60	15 (62.5)	24 (61.5)	21 (56.8)	18 (60.0)	15 (71.4)	27 (55.1)	34 (72.3)	16 (57.1)	10 (40.0)a	27 (51.9)	33 (68.8)
Female	40	9 (37.5)	15 (38.5)	16 (43.2)	12 (40.0)	6 (28.6)	22 (44.9)	13 (27.7)	12 (42.9)	15 (60.0)	25 (48.1)	15 (31.3)
Diameter
< 1.86 ± 1.02	67	16 (66.7)	26 (66.7)	25 (67.6)	15 (50.0)	16 (76.2)	36 (73.5)	28 (59.6)	22 (78.6)	17 (68.0)	38 (73.1)	29 (60.4)
≥ 1.86 ± 1.02	33	8 (33.3)	13 (33.3)	12 (32.4)	15 (50.0)	5 (23.8)	13 (26.5)	19 (40.4)	6 (21.4)	8 (32.0)	14 (26.9)	19 (39.6)
Invasion
T1	7	5 (20.8)	2 (5.1)	0	4 (13.3)	1 (4.8)	2 (4.1)	3 (6.4)	3 (10.7)	1 (4.0)	4 (7.7)	3 (6.3)
T2	28	4 (16.7)	15 (38.5)	9 (24.3)	12 (40.0)	7 (33.3)	9 (18.4)	14 (29.8)	9 (32.1)	5 (20.0)	12 (23.1)	16 (33.3)
T3	42	10 (41.7)	13 (33.3)	19 (51.4)	9 (30.0)	6 (28.6)	27 (55.1)	23 (48.9)	12 (42.9)	7 (28.0)	23 (44.2)	19 (39.6)
T4	23	5 (20.8)	9 (23.1)	9 (24.3)	5 (16.7)	7 (33.3)	11 (22.4)	7 (14.9)	4 (14.3)	12 (48.0)	13 (25.0)	10 (20.8)
LNM
Absent	69	22 (91.7)	29 (74.4)	18 (48.6)b	29 (96.7)	14 (66.7)	26 (53.1)b	39 (83.0)	19 (67.9)	11 (44.0)c	32 (61.5)	37 (77.1)
Present	31	2 (8.3)	10 (25.6)	19 (51.4)	1 (3.3)	7 (33.3)	23 (46.9)	8 (17.0)	9 (32.1)	14 (56.0)	20 (38.5)	11 (22.9)
Metastasis
Absent	79	24 (100.0)	29 (74.4)	26 (70.3)d	21 (70.0)	20 (95.2)	38 (77.6)	39 (83.0)	23 (82.1)	17 (68.0)	40 (76.9)	39 (81.3)
Present	21	0	10 (25.6)	11 (29.7)	9 (30.0)	1 (4.8)	11 (22.4)	8 (17.0)	5 (17.9)	8 (32.0)	12 (23.1)	9 (18.7)
Stage
I	20	11 (45.8)	9 (23.2)	0	10 (33.3)	5 (23.8)	5 (10.2)c	12 (25.5)	6 (21.4)	2 (8.0)	12 (23.1)	8 (16.7)
II	26	6 (25.0)	10 (25.6)	10 (27.0)	7 (23.3)	4 (19.0)	15 (30.6)	15 (31.9)	9 (32.1)	2 (8.0)	12 (23.1)	14 (29.3)
III	32	6 (25.0)	10 (25.6)	16 (43.2)	3 (10.0)	11 (52.4)	18 (36.7)	12 (25.5)	7 (25.0)	13 (52.0)	15 (28.8)	17 (35.2)
IV	22	1 (4.2)	10 (25.6)	11 (29.7)	10 (33.4)	1 (4.8)	11 (22.4)	8 (17.0)	6 (21.4)	8 (32.0)	13 (25.0)	9 (18.8)
Grade
Low	54	19 (79.2)	21 (53.8)	14 (37.8)a	22 (73.3)	12 (57.1)	20 (40.8)a	28 (59.6)	16 (57.1)	10 (40.0)	29 (55.8)	25 (52.1)
Moderate	29	2 (8.3)	13 (33.4)	14 (37.8)	3 (10.0)	6 (28.6)	20 (40.8	11 (23.4)	10 (35.7)	8 (32.0)	15 (28.8)	14 (29.2)
High	17	3 (12.5)	5 (12.8)	9 (24.3)	5 (16.7)	3 (14.3)	9 (18.4)	8 (17.0)	2 (7.2)	7 (28.0)	8 (15.4)	9 (18.7)
LVI
Absent	67	13 (54.2)	26 (66.7)	28 (75.7)	15 (50.0)	15 (71.4)	37 (75.5)	34 (72.3)	14 (50.0)	19 (76.0)	32 (61.5)	35 (72.9)
Present	33	11 (45.8)	13 (33.3)	9 (24.3)	15 (50.0)	6 (28.6)	12 (24.5)	13 (27.7)	14 (50.0)	6 (24.0)	20 (38.5)	13 (27.1)
PNI
Absent	71	14 (58.3)	26 (66.7)	31 (83.8)	18 (60.0)	15 (71.4)	38 (77.6)	34 (72.3)	16 (57.1)	21 (84.0)	37 (71.2)	34 (70.8)
Present	29	10 (41.7)	13 (33.3)	6 (16.2)	12 (40.0)	6 (28.6)	11 (22.4)	13 (27.7)	12 (42.9)	4 (16.0)	15 (28.8)	14 (29.2)
Survival
Deceased	71	7 (29.2)	27 (69.2)	37 (100.0)b	7 (23.3)	16 (76.2)	48 (98.0)b	29 (61.7)	20 (71.4)	22 (88.0)	41 (78.8)	30 (62.5)
Alive	29	17 (70.8)	12 (30.8)	0	23 (76.7)	5 (23.8)	1 (2.0)	18 (38.3)	8 (28.6)	3 (12.0)	11 (21.2)	18 (37.5)
PTB
PTB1	24	-	-	-	17 (56.7)	3 (14.3)	4 (8.2)b	14 (29.8)	8 (28.6)	2 (8.0)c	10 (19.2)	14 (29.2)d
PTB2	39	-	-	-	13 (43.3)	18 (85.7)	8 (16.3)	23 (48.9)	11 (39.3)	5 (20.0)	16 (30.8)	23 (47.9)
PTB3	37	-	-	-	0	0	37 (75.5)	10 (21.3)	9 (32.1)	18 (72.0)	26 (50.0)	11 (22.9)
ITB
ITB1	30	-	-	-	-	-	-	21 (44.7)	8 (28.6)	1 (4.0)c	9 (17.3)	21 (43.8)c
ITB2	21	-	-	-	-	-	-	12 (25.5)	3 (10.7)	6 (24.0)	9 (17.3)	12 (25.0)
ITB3	49	-	-	-	-	-	-	14 (29.8)	17 (60.7)	18 (72.0)	34 (65.4)	15 (31.2)
DR
DR1	47	-	-	-	-	-	-	-	-	-	17 (32.7)	30 (62.5)d
DR2	28	-	-	-	-	-	-	-	-	-	17 (32.7)	11 (22.9)
DR3	25	-	-	-	-	-	-	-	-	-	18 (34.6)	7 (14.6)

^aP < 0.05.

^bP < 0.001.

^cP < 0.008.

^dP < 0.01.

N: Number of cases; PTB: Peritumoral budding; ITB: Intratumoral budding; DR: Desmoplastic reaction; TILs: Tumor-infiltrating lymphocytes; LNM: Lymph node metastasis.