Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry

Figure 1 Imaging mass spectrometry analysis of a gastric mucosa. A: HE staining of the gastric mucosa. Inset, ROI of the imaging analysis; B: Averaged spectra obtained from five gastric mucosae; C: Magnified view of the ROI represented in the inset of (A), HE; D: The ion at m/z 725.5; E: The ion at m/z 780.5; F: The ion at m/z 782.5 were imaged using BioMap.

Figure 2 Ion assignment of m/z 725. 5, 780.5, and 782.5 and immunohistochemical analyses of the gastric mucosae. MS/MS analyses were performed to identify the ions at m/z 725.5 (A), 780.5 (D), and 782.5 (G). The ion images of m/z 725.5 (B), 780.5 (E), and 782.5 (H) are shown using BioMap software. Immunohistochemical analyses were performed using the antibodies against MUC5AC (C), H(+)-K(+)-ATPaseβ (F), and claudin18 (I) in the adjacent specimens used in the imaging MS analyses. Scale bar, 1 mm. MS: Mass spectrometry.