Hydrogen alleviates right ventricular hypertrophy by inhibiting ferroptosis via restoration of the Nrf2/HO-1 signaling pathway

Figure 11  The Nrf2 inhibitor ML385 (1 μmol/L) inhibited restoration of the Nrf2/HO-1 signaling pathway in an angiotensin II-treated H9C2 cells after Hydrogen treatment. A: Cell viability in each group; B: Representative western blotting of Nrf2, TFR1, SLC7A11, HO-1, FTH1, and GPX4 proteins; C: Relative levels of Nrf2, TFR1, SLC7A11, HO-1, FTH1, and GPX4 proteins. Data are expressed as the mean ± SD; n ≥ 3. ^aP < 0.05 and ^cP < 0.01: Ang II group vs control group; ^bP < 0.05 and ^dP < 0.01: Ang II+H₂ group vs Ang II group; ^eP < 0.05 and ^fP < 0.01: Ang II+H₂+ML385 group vs Ang II+H₂ group. MCT: Monocrotaline; H₂: Hydrogen; Ang II: An angiotensin II.