Hydrogen alleviates right ventricular hypertrophy by inhibiting ferroptosis via restoration of the Nrf2/HO-1 signaling pathway

Figure 9 Hydrogen ameliorated oxidative stress and suppresses reactive oxygen species overproduction in H9C2 cells. A: Representative images of the DCFH-DA staining. Magnification is 20 ×. Scale bar = 50 µm; B: Representative images of JC-1 staining. Magnification is 10 ×. Scale bar = 100 µm; C: Relative reactive oxygen species levels in each group; n = 4; D: Relative JC-1 aggregate intensity in each group; n = 4. Data are expressed as the mean ± SD. ^cP < 0.01: Ang II group vs control group; ^dP < 0.01: Ang II+H₂ group vs Ang II group. MCT: Monocrotaline; H₂: Hydrogen; Ang II: An angiotensin II; ROS: Reactive oxygen species.