Hydrogen alleviates right ventricular hypertrophy by inhibiting ferroptosis via restoration of the Nrf2/HO-1 signaling pathway

Figure 8 Hydrogen inhibited an angiotensin II-induced cardiomyocyte hypertrophy in H9C2 cells. A: Representative images of phalloidin staining in H9C2 cells from different groups. Phalloidin and DAPI staining images were merged. Magnification is 20 ×. Scale bar = 50 µm; B: Surface area of H9C2 cells in different groups; n = 4; C: Relative expression levels of ANP mRNA in each group; n = 3; D: Relative BNP mRNA expression in each group; n = 3. Data are expressed as the mean ± SD. ^aP < 0.01 and ^cP < 0.01: Ang II group vs control group; ^dP < 0.01: Ang II+H₂ group vs Ang II group. MCT: Monocrotaline; H₂: Hydrogen; Ang II: An angiotensin II; ANP: Antinatriuretic peptide A; BNP: Brain natriuretic peptide.