Hydrogen alleviates right ventricular hypertrophy by inhibiting ferroptosis via restoration of the Nrf2/HO-1 signaling pathway

Figure 7 Hydrogen alleviates Fe²⁺ content and oxidative stress in an angiotensin II-treated H9C2 cells. A: Cell viability with different An angiotensin II concentrations (0, 0.01, 0.1, 1, 10, and 100 μmol/L) relative to the control; B: Relative cell viability in each group; C: Relative lactate dehydrogenase release in each group; D: Relative Fe²⁺ content in each group, n = 4; E: Relative malonaldehyde content in each group; n = 4; F: Relative total superoxide dismutase activity in each group; n = 4; G: Relative glutathione content of each group; n = 4; H: Relative GSH-Px activity in each group; n = 4. Data are expressed as the mean ± SD. ^cP < 0.01: Ang II group vs control group; ^bP < 0.05 and ^dP < 0.01: Ang II+H₂ group vs Ang II group. MCT: Monocrotaline; H₂: Hydrogen; MDA: Malonaldehyde; LDH: lactate dehydrogenase; T-SOD: total superoxide dismutase; Ang II: An angiotensin II.