Hydrogen alleviates right ventricular hypertrophy by inhibiting ferroptosis via restoration of the Nrf2/HO-1 signaling pathway

Figure 6 Hydrogen inhalation alleviated ferroptosis by restoring the Nrf2/HO-1 pathway in monocrotaline-treated rats. A: Representative images of immunohistochemistry (IHC) staining of Nrf2. Nrf2-positive cell nuclei are stained brown. Magnification is 20 ×. Scale bar = 50 µm; B: Representative images of IHC staining of HO-1. HO-1-positive area is stained brown. Magnification is 20 ×. Scale bar = 50 µm; C: Quantitative analysis of IHC staining of Nrf2; n = 4; D: Quantitative analysis of IHC staining of HO-1; n = 4; E: Representative western blotting of Nrf2, TFR1, SLC7A11, HO-1, FTH1 and GPX4 proteins; F: Relative levels of Nrf2, TFR1, SLC7A11, HO-1, FTH1, and GPX4 proteins. Data are expressed as the mean ± SD; n ≥ 3. ^aP < 0.05 and ^cP < 0.01: MCT group vs control group; ^bP < 0.05 and ^dP < 0.01: MCT+H₂ group vs MCT group. MCT: Monocrotaline; H₂: Hydrogen.