Hydrogen alleviates right ventricular hypertrophy by inhibiting ferroptosis via restoration of the Nrf2/HO-1 signaling pathway

Figure 3 Hydrogen inhalation further reduced the expression of hypertrophy-related indicators in monocrotaline-treated rats. A: Representative images of immunohistochemistry (IHC) staining of Antinatriuretic peptide A (ANP). ANP proteins were stained brown. Magnification is 20 ×. Scale bar = 50 µm; B: Representative images of IHC staining of brain natriuretic peptide (BNP). BNP proteins were stained brown. Magnification is 20 ×. Scale bar = 50 µm; C: Quantitative analysis of IHC staining of ANP; n = 4; D: Quantitative analysis of IHC staining of BNP; n = 4; E and F: Representative western blotting bands of ANP and BNP proteins and their relative levels; n = 3. Data are expressed as the mean ± SD. ^cP < 0.01: MCT group vs control group; ^bP < 0.05 and ^dP < 0.01: MCT+H₂ group vs MCT group. ANP: Antinatriuretic peptide A; BNP: Brain natriuretic peptide; MCT: Monocrotaline; H₂: Hydrogen.