GRK5 is an essential co-repressor of the cardiac mineralocorticoid receptor and is selectively induced by finerenone

Figure 3 Comparison of anti-apoptotic and anti-oxidative efficacies of finerenone vs eplerenone in aldosterone-treated cardiomyocytes in the presence or absence of G protein-coupled receptor-kinase 5. A: Apoptotic cell death in control, empty vector (mock) virus-infected (EV) cells or in cells having G protein-coupled receptor-kinase (GRK)-5 genetically (CRISPR lentivirus-mediated) deleted (GRK5-KO) and treated with 100 nmol/L aldosterone (Aldo) alone or in the presence of 10 mmol/L eplerenone (Epl) or 10 mmol/L finerenone for 24 h. ^aP < 0.05, vs Epl; n = 4 independent experiments per transfection/treatment. Essentially no inhibition of Aldo-induced apoptosis could be detected with either drug in GRK5-KO cells; B: ROS generation in these cells, as measured via the DCF assay. ^aP < 0.05. Not significant at P = 0.05; n = 4 independent experiments per transfection/treatment. EV: Empty virus; Epl: Eplerenone; Fin: Finerenone; NS: Not significant.