Inhibitory role of TACE/ADAM17 cytotail in protein ectodomain shedding

Figure 3 Deletion of the cytotail from C600Y tumor necrosis factor-α converting enzyme led to partial restoration of L-selectin and transforming growth factor α shedding. Expression plasmids for wild-type or mutated tumor necrosis factor-α converting enzyme (TACE) are schematically shown on the top of the Figure. The activity of L-selectin shedding in the TACE constructs was determined as in Figure 2. Transforming growth factor (TGF)-α shedding was determined in a similar manner except the M1-L-selectin cells were replaced with shedding-defective M1-TGF-α cells, which overexpress transmembrane TGF-α, and the anti-L-selectin was substituted with anti-TGF-α.