Ras homolog enriched in brain 1 regulates β cell mass and β cell function via mTORC1/AMPK/Notch1 pathways

Figure 1 Rheb1 upregulates β cell mass and mTORC1 signaling. A: Rheb1 expression in the islets isolated from people with different age; B: Immunofluorescence staining of Rheb1 (red) and insulin (as a β-cell marker; green) in pancreatic sections of 8-week-old male pRheb1KO mice (n = 3) and wild-type (WT) mice (n = 3). Scale bars: 10 μm; C and D: Average β-cell mass and β-cell size in pancreatic sections of 2-month-old male pRheb1KO (n = 4) and WT mice (n = 5) subjected to insulin staining; E: Anti-apoptotic genes from RNA-seq of islets of bRheb1KO mice (n = 3) and WT mice (n = 3), of which is reanalyzed from our previous RNA-seq data[3]. All data are represented as the mean ± SD, ^aP < 0.05, ^bP < 0.01. WT: Wild-type.