miR-4770 promotes colorectal cancer progression by targeting PRKAA2 via m6A modification

Figure 5 Expression of miR-4770 in colorectal cancer cells and its regulation of PRKAA2. A: The mRNA levels of miR-4770, METTL3 and PRKAA2 in FHC, HCT116 and SW480 cells were detected by qRT-PCR; B and C: In SW480 cells, qRT-PCR was used to detect the expression of miR-4770 (B) and PRKAA2 (C) in each group, which were divided into NC inhibitor, miR inhibitor, NC mimic and miR mimic; D: Western blot was used to detect the level of PRKAA2 protein in the above four groups of SW480 cells. Data are expressed as mean ± SD and are derived from three independent biological replicates. Statistical difference: ^aP < 0.01 vs FHC group; ^bP < 0.05 and ^cP < 0.01 vs NC inhibitor group; ^dP < 0.01 vs NC mimic group.