miR-4770 promotes colorectal cancer progression by targeting PRKAA2 via m6A modification

Figure 4 miR-4770 regulates the viability, migration and invasion of HCT116 cells through PRKAA2. A: QRT-PCR was used to detect the mRNA expression of PRKAA2 in siNC group and si-PRKAA2 group; B: CCK-8 method was used to detect the proliferation activity of four groups of cells at 24 hours, 48 hours and 72 hours: NC inhibitor + siNC, miR inhibitor + siNC, NC inhibitor + si-PRKAA2, miR inhibitor + si-PRKAA2; C and D: Transwell assay was used to evaluate the migration (C) and invasion (D) abilities of the above four groups of cells. Scale: 100 μm. The data are expressed as mean ± SD and are derived from three independent biological replicates. ^aP < 0.01 vs siNC group; ^bP < 0.05 and ^cP < 0.01 vs NC inhibitor + siNC group; ^dP < 0.05 and ^eP < 0.01 vs miR inhibitor + siNC group; ^fP < 0.05 and ^gP < 0.01 vs NC inhibitor + si-PRKAA2 group.