miR-4770 promotes colorectal cancer progression by targeting PRKAA2 via m6A modification

Figure 3 Verification of the targeting relationship between PRKAA2 and miR-4770. A: The mRNA levels of PRKAA2 in colorectal cancer (CRC) tissues and normal tissues were determined by qRT-PCR; B: The expression of the two in CRC tissues was negatively correlated; C: The expression of PRKAA2 was detected by qRT-PCR in four groups of cells: NC inhibitor, miR inhibitor, NC mimic and miR mimic; D: Western blot was used to detect the level of PRKAA2 protein in the above four groups of cells; E: Predicting the target binding sequence of miR-4770 and PRKAA2; F: Dual luciferase reporter assay verified the direct targeting relationship between the two. Data were expressed as mean ± SD. ^aP < 0.01 vs normal group; ^bP < 0.01 vs NC inhibitor group; ^cP < 0.05 vs NC mimic group; ^dP < 0.01 vs miR-4770 NC group. Clinical tissue analyses included 10 paired CRC and adjacent normal tissues, and in vitro experiments were performed with three independent biological replicates. CRC: Colorectal cancer.